Qiangfu Hu1, Dan Zhou2, Xiaopei Li1, Nan Yang1, Peilei Guo1, Dengfei Xu1, Xia Li1. 1. Department of Anesthesiology, Fifth Affiliated Hospital of Zhengzhou University Kangfuqian Street, Zhengzhou 450052, Henan, P. R. China. 2. Department of Cardiac Surgery, Wuhan Asia Heart Hospital 753 Jianshe Street, Wuhan 430022, Hubei, P. R. China.
Abstract
AIMS: This study aims to explore the pathogenesis of myocardial ischemia-reperfusion injury and the treatment method. METHODS: Myocardial Ischemia-reperfusion rat model was established in this study. They were divided into three groups: sham operation (SO) group, IRI control (C) group and IRI with propofol (A) group (n = 9). Myocardial infarct size was compared with HE staining method. TUNEL assay was used to detect cell apoptosis. Changes in the expression of iNOS were detected using real-time PCR and Western blotting methods. RESULTS: Myocardial infarct size of control group and propofol group was 53.03 ± 8.90% and 34.73 ± 7.20% respectively, there were significant differences between them (P < 0.01). Apoptotic index of two groups was 0.21 ± 0.02 and 0.31 ± 0.05, with statistical significance (P < 0.05). The expression levels of iNOS in propofol group reduced significantly (P < 0.05). CONCLUSION: The levels of iNOS increase in IRI rats, suggesting that the severity of myocardial failure may be correlated with iNOS; propofol can specifically inhibit iNOS and thus protect the myocardial function.
AIMS: This study aims to explore the pathogenesis of myocardial ischemia-reperfusion injury and the treatment method. METHODS: Myocardial Ischemia-reperfusion rat model was established in this study. They were divided into three groups: sham operation (SO) group, IRI control (C) group and IRI with propofol (A) group (n = 9). Myocardial infarct size was compared with HE staining method. TUNEL assay was used to detect cell apoptosis. Changes in the expression of iNOS were detected using real-time PCR and Western blotting methods. RESULTS:Myocardial infarct size of control group and propofol group was 53.03 ± 8.90% and 34.73 ± 7.20% respectively, there were significant differences between them (P < 0.01). Apoptotic index of two groups was 0.21 ± 0.02 and 0.31 ± 0.05, with statistical significance (P < 0.05). The expression levels of iNOS in propofol group reduced significantly (P < 0.05). CONCLUSION: The levels of iNOS increase in IRI rats, suggesting that the severity of myocardial failure may be correlated with iNOS; propofol can specifically inhibit iNOS and thus protect the myocardial function.