F H Heida1, J B F Hulscher2, M Schurink2, A Timmer3, E M W Kooi4, A F Bos4, J L M Bruggink2, D C Kasper5, M Pones6, T Benkoe6. 1. Department of Pediatric Surgery, Beatrix Children's Hospital, University of Groningen, University Medical Center Groningen, The Netherlands. Electronic address: f.h.heida@umcg.nl. 2. Department of Pediatric Surgery, Beatrix Children's Hospital, University of Groningen, University Medical Center Groningen, The Netherlands. 3. Department of Pediatric Pathology, Beatrix Children's Hospital, University of Groningen, University Medical Center Groningen, The Netherlands. 4. Department of Pediatric Neonatology, Beatrix Children's Hospital, University of Groningen, University Medical Center Groningen, The Netherlands. 5. Research Core Unit for Pediatric Biochemistry and Analytics, Medical University of Vienna, Austria. 6. Department of Pediatric Surgery, Medical University of Vienna, Austria.
Abstract
BACKGROUND: Intestinal fatty acid-binding protein (I-FABP) is considered as a specific marker for enterocyte damage in necrotizing enterocolitis (NEC). OBJECTIVE: The purpose of this study was to evaluate the association of plasma and urinary I-FABP levels with the extent of macroscopic intestinal necrosis in surgical NEC. METHODS: We combined data from prospective trials from two large academic pediatric surgical centers. Nine and 10 infants with surgical NEC were included, respectively. Plasma and urinary of I-FABP at disease onset were correlated with the length of intestinal resection during laparotomy. RESULTS: Median length of bowel resection was 10cm (range 2.5-50) and 17cm (range 0-51), respectively. Median I-FABP levels were 53ng/mL (range 6.3-370) and 4.2ng/mL (range 1.1-15.4) in plasma in cohort 1 respectively cohort 2 and 611ng/mL (range 3-23,336) in urine. The length of bowel resection significantly correlated with I-FABP levels in plasma (Rho 0.68; p=0.04 and Rho 0.66;p=0.04) and in urine (Rho 0.92; p=0.001). CONCLUSION: This 'proof of concept' study demonstrates that plasma and urine I-FABP levels at disease onset was strongly associated with the length of intestinal resection in surgical NEC. This offers further evidence that I-FABP levels are a promising biomarker for assessing intestinal necrosis in infants with advanced NEC.
BACKGROUND:Intestinal fatty acid-binding protein (I-FABP) is considered as a specific marker for enterocyte damage in necrotizing enterocolitis (NEC). OBJECTIVE: The purpose of this study was to evaluate the association of plasma and urinary I-FABP levels with the extent of macroscopic intestinal necrosis in surgical NEC. METHODS: We combined data from prospective trials from two large academic pediatric surgical centers. Nine and 10 infants with surgical NEC were included, respectively. Plasma and urinary of I-FABP at disease onset were correlated with the length of intestinal resection during laparotomy. RESULTS: Median length of bowel resection was 10cm (range 2.5-50) and 17cm (range 0-51), respectively. Median I-FABP levels were 53ng/mL (range 6.3-370) and 4.2ng/mL (range 1.1-15.4) in plasma in cohort 1 respectively cohort 2 and 611ng/mL (range 3-23,336) in urine. The length of bowel resection significantly correlated with I-FABP levels in plasma (Rho 0.68; p=0.04 and Rho 0.66;p=0.04) and in urine (Rho 0.92; p=0.001). CONCLUSION: This 'proof of concept' study demonstrates that plasma and urine I-FABP levels at disease onset was strongly associated with the length of intestinal resection in surgical NEC. This offers further evidence that I-FABP levels are a promising biomarker for assessing intestinal necrosis in infants with advanced NEC.
Authors: Takeshi Kitai; Yong-Hyun Kim; Kathryn Kiefer; Rommel Morales; Allen G Borowski; Justin L Grodin; W H Wilson Tang Journal: Clin Biochem Date: 2017-02-20 Impact factor: 3.281
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