Tian Liu1, Shilong Zhong2, Fang Rao2, Yumei Xue1, Zhoucuo Qi2, Shulin Wu3. 1. Department of Cardiology, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China. 2. Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Medical Research Center of Guangdong General Hospital, Guangzhou, Guangdong 510100, China. 3. Department of Cardiology, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China wushull@126.com.
Abstract
AIMS: Despite numerous studies identifying specific microRNA (miRNA) expression profiles associated with atrial fibrillation (AF), changes in plasma miRNA expression in pre- and post-operative AF patients who have received catheter ablation, remain poorly characterized. This study aimed to reveal disease-related biomarkers by detecting plasma miRNA expression in AF patients, and examining the levels of AF-specific miRNAs in patients after catheter ablation, in order to help gauge therapeutic effects and assess prognosis. METHODS AND RESULTS: A total of 100 Han Chinese patients with AF who had received catheter ablation, and 100 healthy individuals, were sequentially recruited to the study. Atrial fibrillation-specific plasma miRNAs were detected by Solexa sequencing and quantitative reverse transcription polymerase chain reaction. The expression levels of AF-specific miRNAs were also investigated in 40 post-operative patients (24-48 h) and 20 patients followed up (58.52 ± 36.00 days) after catheter ablation, to explore changes in miRNA expression. The expressions of miR-409-3p and miR-432 in the plasma of AF patients were lower than healthy individuals. In binary logistic regression analyses, reduced miR-409-3p and miR-432 levels were independently associated with AF (95% confidence interval: 1.02-2.22 and 1.09-2.43, P = 0.040 and 0.018, respectively). The levels of miR-409-3p and miR-432 showed no significant difference between post-operative patients and healthy individuals (P = 0.411 and 0.681, respectively), or between followed-up patients and healthy individuals (P = 0.720 and 0.073, respectively). CONCLUSION: We suggest that plasma miR-409-3p and miR-432 are potential markers of AF, and catheter ablation restores their decreased levels in AF patients. Published on behalf of the European Society of Cardiology. All rights reserved.
RCT Entities:
AIMS: Despite numerous studies identifying specific microRNA (miRNA) expression profiles associated with atrial fibrillation (AF), changes in plasma miRNA expression in pre- and post-operative AFpatients who have received catheter ablation, remain poorly characterized. This study aimed to reveal disease-related biomarkers by detecting plasma miRNA expression in AFpatients, and examining the levels of AF-specific miRNAs in patients after catheter ablation, in order to help gauge therapeutic effects and assess prognosis. METHODS AND RESULTS: A total of 100 Han Chinese patients with AF who had received catheter ablation, and 100 healthy individuals, were sequentially recruited to the study. Atrial fibrillation-specific plasma miRNAs were detected by Solexa sequencing and quantitative reverse transcription polymerase chain reaction. The expression levels of AF-specific miRNAs were also investigated in 40 post-operative patients (24-48 h) and 20 patients followed up (58.52 ± 36.00 days) after catheter ablation, to explore changes in miRNA expression. The expressions of miR-409-3p and miR-432 in the plasma of AFpatients were lower than healthy individuals. In binary logistic regression analyses, reduced miR-409-3p and miR-432 levels were independently associated with AF (95% confidence interval: 1.02-2.22 and 1.09-2.43, P = 0.040 and 0.018, respectively). The levels of miR-409-3p and miR-432 showed no significant difference between post-operative patients and healthy individuals (P = 0.411 and 0.681, respectively), or between followed-up patients and healthy individuals (P = 0.720 and 0.073, respectively). CONCLUSION: We suggest that plasma miR-409-3p and miR-432 are potential markers of AF, and catheter ablation restores their decreased levels in AFpatients. Published on behalf of the European Society of Cardiology. All rights reserved.
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