Qiuying Shi 1 , Ashley Ibrahim 1 , Kristi Herbert 1 , Marcia Carvin 1 , Melissa Randolph 1 , Kristin M Post 1 , Kendra Curless 1 , Shaoxiong Chen 1 , Harvey M Cramer 1 , Liang Cheng 1 , Howard H Wu 2 Show Affiliations »
Abstract
OBJECTIVES: To determine the utility of the cell transfer technique (CTT) for BRAF molecular testing on thyroid fine-needle aspiration (FNA) specimens. METHODS: Polymerase chain reaction (PCR)-based BRAF molecular testing was performed on tissues obtained through CTT from both air-dried and ethanol-fixed direct smears of thyroid FNA specimens and then compared with the corresponding thyroidectomy formalin-fixed, paraffin-embedded (FFPE) tissues on 30 cases. RESULTS: BRAF testing was successfully performed on 29 of 30 air-dried CTT, 27 of 30 ethanol-fixed CTT, and 27 of 30 FFPE tissues. The results exhibited 11, 13, and 13 BRAF mutations and 18, 14, and 14 wild types for the air-dried CTT, the ethanol-fixed CTT, and the FFPE tissues, respectively. The concordance rate was 96% between air-dried and ethanol-fixed CTT tissues, 88% between air-dried CTT and FFPE tissues, and 92% between ethanol-fixed CTT and FFPE tissues. CONCLUSIONS: PCR-based BRAF mutational testing can be reliably performed on the direct smears of the thyroid FNA specimens through the application of CTT. Copyright© by the American Society for Clinical Pathology.
OBJECTIVES: To determine the utility of the cell transfer technique (CTT ) for BRAF molecular testing on thyroid fine-needle aspiration (FNA) specimens. METHODS: Polymerase chain reaction (PCR)-based BRAF molecular testing was performed on tissues obtained through CTT from both air-dried and ethanol -fixed direct smears of thyroid FNA specimens and then compared with the corresponding thyroidectomy formalin -fixed, paraffin -embedded (FFPE) tissues on 30 cases. RESULTS: BRAF testing was successfully performed on 29 of 30 air-dried CTT , 27 of 30 ethanol -fixed CTT , and 27 of 30 FFPE tissues. The results exhibited 11, 13, and 13 BRAF mutations and 18, 14, and 14 wild types for the air-dried CTT , the ethanol -fixed CTT , and the FFPE tissues, respectively. The concordance rate was 96% between air-dried and ethanol -fixed CTT tissues, 88% between air-dried CTT and FFPE tissues, and 92% between ethanol -fixed CTT and FFPE tissues. CONCLUSIONS: PCR-based BRAF mutational testing can be reliably performed on the direct smears of the thyroid FNA specimens through the application of CTT . Copyright© by the American Society for Clinical Pathology.
Entities: Chemical
Gene
Keywords:
BRAF mutation; Cell transfer; Cytology; Molecular; Thyroid
Mesh： See more »
Substances： See more »
Year: 2015
PMID： 25780001 DOI： 10.1309/AJCP5BG0KUEOJCVS
Source DB: PubMed Journal: Am J Clin Pathol ISSN： 0002-9173 Impact factor: 2.493