Literature DB >> 25779315

Construction of conditional knockdown mutants in mycobacteria.

Dirk Schnappinger1, Kathryn M O'Brien, Sabine Ehrt.   

Abstract

By definition, essential genes are fundamental to bacterial growth, yet the functions of many such genes remain unknown. Essential genes furthermore are central to the activity of most antibacterial drugs and among the most attractive targets for the development of new therapeutics. This chapter describes how synthetic genetic switches that utilize transcriptional repression, controlled proteolysis, or both to silence gene activity can be applied to construct and characterize conditional knockdown (cKD) mutants for essential genes in Mycobacterium smegmatis and Mycobacterium tuberculosis.

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Year:  2015        PMID： 25779315     DOI： 10.1007/978-1-4939-2450-9_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  20 in total

1.  Phosphorylation on PstP Regulates Cell Wall Metabolism and Antibiotic Tolerance in Mycobacterium smegmatis.

Authors:  Farah Shamma; Kadamba Papavinasasundaram; Samantha Y Quintanilla; Aditya Bandekar; Christopher Sassetti; Cara C Boutte
Journal:  J Bacteriol       Date:  2021-01-25       Impact factor: 3.490

Review 2.  New agents for the treatment of drug-resistant Mycobacterium tuberculosis.

Authors:  Daniel T Hoagland; Jiuyu Liu; Robin B Lee; Richard E Lee
Journal:  Adv Drug Deliv Rev       Date:  2016-05-02       Impact factor: 15.470

3.  Investigation of ( S)-(-)-Acidomycin: A Selective Antimycobacterial Natural Product That Inhibits Biotin Synthase.

Authors:  Matthew R Bockman; Curtis A Engelhart; Julia D Cramer; Michael D Howe; Neeraj K Mishra; Matthew Zimmerman; Peter Larson; Nadine Alvarez-Cabrera; Sae Woong Park; Helena I M Boshoff; James M Bean; Victor G Young; David M Ferguson; Veronique Dartois; Joseph T Jarrett; Dirk Schnappinger; Courtney C Aldrich
Journal:  ACS Infect Dis       Date:  2019-02-04       Impact factor: 5.084

4.  Opposing reactions in coenzyme A metabolism sensitize Mycobacterium tuberculosis to enzyme inhibition.

Authors:  Elaine Ballinger; John Mosior; Travis Hartman; Kristin Burns-Huang; Ben Gold; Roxanne Morris; Laurent Goullieux; Isabelle Blanc; Julien Vaubourgeix; Sophie Lagrange; Laurent Fraisse; Stéphanie Sans; Cedric Couturier; Eric Bacqué; Kyu Rhee; Sarah M Scarry; Jeffrey Aubé; Guangbin Yang; Ouathek Ouerfelli; Dirk Schnappinger; Thomas R Ioerger; Curtis A Engelhart; Jennifer A McConnell; Kathrine McAulay; Allison Fay; Christine Roubert; James Sacchettini; Carl Nathan
Journal:  Science       Date:  2019-02-01       Impact factor: 47.728

5.  Structural and Genetic Analyses of the Mycobacterium tuberculosis Protein Kinase B Sensor Domain Identify a Potential Ligand-binding Site.

Authors:  Daniil M Prigozhin; Kadamba G Papavinasasundaram; Christina E Baer; Kenan C Murphy; Alisa Moskaleva; Tony Y Chen; Tom Alber; Christopher M Sassetti
Journal:  J Biol Chem       Date:  2016-09-06       Impact factor: 5.157

6.  Therapeutic Potential of the Mycobacterium tuberculosis Mycolic Acid Transporter, MmpL3.

Authors:  Wei Li; Andrés Obregón-Henao; Joshua B Wallach; E Jeffrey North; Richard E Lee; Mercedes Gonzalez-Juarrero; Dirk Schnappinger; Mary Jackson
Journal:  Antimicrob Agents Chemother       Date:  2016-08-22       Impact factor: 5.191

7.  Mycobacterium tuberculosis Thioredoxin Reductase Is Essential for Thiol Redox Homeostasis but Plays a Minor Role in Antioxidant Defense.

Authors:  Kan Lin; Kathryn M O'Brien; Carolina Trujillo; Ruojun Wang; Joshua B Wallach; Dirk Schnappinger; Sabine Ehrt
Journal:  PLoS Pathog       Date:  2016-06-01       Impact factor: 6.823

8.  Horizontal acquisition of a hypoxia-responsive molybdenum cofactor biosynthesis pathway contributed to Mycobacterium tuberculosis pathoadaptation.

Authors:  Florence Levillain; Yannick Poquet; Ludovic Mallet; Serge Mazères; Michael Marceau; Roland Brosch; Franz-Christoph Bange; Philip Supply; Axel Magalon; Olivier Neyrolles
Journal:  PLoS Pathog       Date:  2017-11-27       Impact factor: 6.823

9.  Depleting Mycobacterium tuberculosis of the transcription termination factor Rho causes pervasive transcription and rapid death.

Authors:  Laure Botella; Julien Vaubourgeix; Jonathan Livny; Dirk Schnappinger
Journal:  Nat Commun       Date:  2017-03-28       Impact factor: 14.919

10.  Identification of Enolase as the Target of 2-Aminothiazoles in Mycobacterium tuberculosis.

Authors:  Heather H Wescott; Edison S Zuniga; Anumita Bajpai; Carolina Trujillo; Sabine Ehrt; Dirk Schnappinger; David M Roberts; Tanya Parish
Journal:  Front Microbiol       Date:  2018-10-26       Impact factor: 5.640
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