Literature DB >> 25779313

Electroporation of mycobacteria.

Renan Goude1, David M Roberts, Tanya Parish.   

Abstract

High-efficiency transformation of DNA is integral to the study of mycobacteria, allowing genetic manipulation. Electroporation is the most widely used method for introducing DNA into mycobacterial strains. Many parameters contribute to high-efficiency transformation; these include the species per strain, the transforming DNA, the selectable marker, the growth medium additives, and the conditions of electroporation. In this chapter we provide an optimized method for the transformation of representative slow- and fast-growing species of mycobacteria-Mycobacterium tuberculosis and M. smegmatis, respectively.

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Year:  2015        PMID： 25779313     DOI： 10.1007/978-1-4939-2450-9_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  21 in total

1.  Conserved ESX-1 Substrates EspE and EspF Are Virulence Factors That Regulate Gene Expression.

Authors:  Alexandra E Chirakos; Kathleen R Nicholson; Allison Huffman; Patricia A Champion
Journal:  Infect Immun       Date:  2020-11-16       Impact factor: 3.441

2.  Delineating the Physiological Roles of the PE and Catalytic Domains of LipY in Lipid Consumption in Mycobacterium-Infected Foamy Macrophages.

Authors:  Pierre Santucci; Sadia Diomandé; Isabelle Poncin; Laetitia Alibaud; Albertus Viljoen; Laurent Kremer; Chantal de Chastellier; Stéphane Canaan
Journal:  Infect Immun       Date:  2018-08-22       Impact factor: 3.441

3.  Hypoxanthine-Guanine Phosphoribosyltransferase Is Dispensable for Mycobacterium smegmatis Viability.

Authors:  Zdeněk Knejzlík; Klára Herkommerová; Dana Hocková; Iva Pichová
Journal:  J Bacteriol       Date:  2020-02-11       Impact factor: 3.490

4.  PhdA Catalyzes the First Step of Phenazine-1-Carboxylic Acid Degradation in Mycobacterium fortuitum.

Authors:  Kyle C Costa; Leon S Moskatel; Lucas A Meirelles; Dianne K Newman
Journal:  J Bacteriol       Date:  2018-04-24       Impact factor: 3.490

5.  A Mycobacterium avium subsp. paratuberculosis Predicted Serine Protease Is Associated with Acid Stress and Intraphagosomal Survival.

Authors:  Abirami Kugadas; Elise A Lamont; John P Bannantine; Fernanda M Shoyama; Evan Brenner; Harish K Janagama; Srinand Sreevatsan
Journal:  Front Cell Infect Microbiol       Date:  2016-08-22       Impact factor: 5.293

6.  Imaging the NADH:NAD+ Homeostasis for Understanding the Metabolic Response of Mycobacterium to Physiologically Relevant Stresses.

Authors:  Shabir A Bhat; Iram K Iqbal; Ashwani Kumar
Journal:  Front Cell Infect Microbiol       Date:  2016-11-08       Impact factor: 5.293

7.  Imidazoles Induce Reactive Oxygen Species in Mycobacterium tuberculosis Which Is Not Associated with Cell Death.

Authors:  Heather A Howell Wescott; David M Roberts; Christian L Allebach; Rachel Kokoczka; Tanya Parish
Journal:  ACS Omega       Date:  2017-01-05

8.  Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice.

Authors:  Alissa C Rothchild; Dat Mai; Alan Aderem; Alan H Diercks
Journal:  Bio Protoc       Date:  2020-05-20

9.  Bioenergetic Heterogeneity in Mycobacterium tuberculosis Residing in Different Subcellular Niches.

Authors:  Ajit Kumar Akela; Ashwani Kumar
Journal:  mBio       Date:  2021-06-01       Impact factor: 7.867

10.  A uniform cloning platform for mycobacterial genetics and protein production.

Authors:  Fabian M Arnold; Michael Hohl; Sille Remm; Hendrik Koliwer-Brandl; Sophia Adenau; Sasitorn Chusri; Peter Sander; Hubert Hilbi; Markus A Seeger
Journal:  Sci Rep       Date:  2018-06-22       Impact factor: 4.379
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