| Literature DB >> 2577733 |
R A Floyd1, J J Watson, P K Wong, D H Altmiller, R C Rickard.
Abstract
DNA or 2-deoxyguanosine reacts with hydroxyl free radical to form 8-hydroxy-deoxyguanosine (8-OH-dG). We found that 8-OH-dG can be effectively separated from deoxyguanosine by high pressure liquid chromatography and very sensitively detected using electrochemical detection. The sensitivity of electrochemical detection is about one-thousand fold enhanced over optical detection. Utilizing deoxyguanosine in bicarbonate buffer it was found that ferrous ion, but not ferric ion, was effective in forming 8-OH-dG. The hydroxyl free radical scavenging agents, thiourea and ethanol, were very effective in quenching Fe(11) mediated 8-OH-dG formation, but superoxide dismutase had very little effect.Entities:
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Year: 1986 PMID： 2577733 DOI： 10.3109/10715768609083148
Source DB: PubMed Journal: Free Radic Res Commun ISSN： 8755-0199