Bettina Pyndt Jørgensen1, Gudrun Winther2, Pernille Kihl1, Dennis S Nielsen3, Gregers Wegener2, Axel K Hansen1, Dorte B Sørensen1. 1. 1Section of Experimental Animal Models,Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences,University of Copenhagen,Frederiksberg C,Denmark. 2. 2Translational Neuropsychiatry Unit,Department of Clinical Medicine,Aarhus University,Risskov,Denmark. 3. 3Department of Food Science, Faculty of Science,University of Copenhagen,Frederiksberg C,Denmark.
Abstract
OBJECTIVE: Magnesium deficiency has been associated with anxiety in humans, and rodent studies have demonstrated the gut microbiota to impact behaviour. METHODS: We investigated the impact of 6 weeks of dietary magnesium deficiency on gut microbiota composition and anxiety-like behaviour and whether there was a link between the two. A total of 20 C57BL/6 mice, fed either a standard diet or a magnesium-deficient diet for 6 weeks, were tested using the light-dark box anxiety test. Gut microbiota composition was analysed by denaturation gradient gel electrophoresis. RESULTS: We demonstrated that the gut microbiota composition correlated significantly with the behaviour of dietary unchallenged mice. A magnesium-deficient diet altered the gut microbiota, and was associated with altered anxiety-like behaviour, measured by decreased latency to enter the light box. CONCLUSION: Magnesium deficiency altered behavior. The duration of magnesium deficiency is suggested to influence behaviour in the evaluated test.
OBJECTIVE:Magnesium deficiency has been associated with anxiety in humans, and rodent studies have demonstrated the gut microbiota to impact behaviour. METHODS: We investigated the impact of 6 weeks of dietary magnesium deficiency on gut microbiota composition and anxiety-like behaviour and whether there was a link between the two. A total of 20 C57BL/6 mice, fed either a standard diet or a magnesium-deficient diet for 6 weeks, were tested using the light-dark box anxiety test. Gut microbiota composition was analysed by denaturation gradient gel electrophoresis. RESULTS: We demonstrated that the gut microbiota composition correlated significantly with the behaviour of dietary unchallenged mice. A magnesium-deficient diet altered the gut microbiota, and was associated with altered anxiety-like behaviour, measured by decreased latency to enter the light box. CONCLUSION:Magnesiumdeficiency altered behavior. The duration of magnesium deficiency is suggested to influence behaviour in the evaluated test.
Entities:
Keywords:
animal models; anxiety; gut microbiota; magnesium
Authors: Wolfgang Marx; Melissa Lane; Meghan Hockey; Hajara Aslam; Michael Berk; Ken Walder; Alessandra Borsini; Joseph Firth; Carmine M Pariante; Kirsten Berding; John F Cryan; Gerard Clarke; Jeffrey M Craig; Kuan-Pin Su; David Mischoulon; Fernando Gomez-Pinilla; Jane A Foster; Patrice D Cani; Sandrine Thuret; Heidi M Staudacher; Almudena Sánchez-Villegas; Husnain Arshad; Tasnime Akbaraly; Adrienne O'Neil; Toby Segasby; Felice N Jacka Journal: Mol Psychiatry Date: 2020-11-03 Impact factor: 15.992