Literature DB >> 25773201

Combining tape stripping and non-invasive reflectance confocal microscopy : an in vivo model to study skin damage.

M Peppelman1, W A J van den Eijnde1, E J Jaspers1, M-J P Gerritsen1, P E J van Erp1.   

Abstract

BACKGROUND: Evaluation of (immuno)histological and cell biological changes in damaged skin requires often an invasive skin biopsy, making in vivo models inappropriate to study skin damage. Reflectance confocal microscopy (RCM) might overcome this limitation. Therefore, we evaluated the use of a tape-stripping model in combination with RCM to provide morphological data on skin damage and recovery.
METHODS: In 25 volunteers, a tape-stripping stimulus was applied. The skin was imaged with RCM during 1 week and 3 mm punch biopsies were obtained.
RESULTS: Strong correlations between epidermal thickness determined by RCM and conventional histological measurements were found. RCM thickness measurements correlated well with epidermal proliferation. The 10× or 15× repeated tape-stripping resulted in skin damage similar to acute stripping. Mild repeated tape-stripping showed no skin damage.
CONCLUSION: Overall, we demonstrated that non-invasive RCM in combination with tape-stripping could be used as model to obtain morphological and cell biological data on skin-material interactions.
© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Entities:  

Keywords:  epidermal proliferation and thickness; immunohistochemistry; in vivo models; reflectance confocal microscopy; skin damage; tape stripping

Mesh：

Year:  2015        PMID： 25773201     DOI： 10.1111/srt.12217

Source DB:  PubMed          Journal:  Skin Res Technol        ISSN： 0909-752X            Impact factor:   2.365


  7 in total

1.  Comparison of the Effect of Skin Preparation Pads on Transepidermal Water Loss in Ex Vivo Human Skin.

Authors:  Hanan Osman-Ponchet; Alexandre Gaborit; Magali Kouidhi; Sandrine Anglars; Jeanne Marceau-Suissa; Orla Duffy-Roger; Jean-Michel Linget; Claire E Wilson
Journal:  Dermatol Ther (Heidelb)       Date:  2017-07-14

2.  A 3D self-organizing multicellular epidermis model of barrier formation and hydration with realistic cell morphology based on EPISIM.

Authors:  Thomas Sütterlin; Erika Tsingos; Jalil Bensaci; Georgios N Stamatas; Niels Grabe
Journal:  Sci Rep       Date:  2017-03-06       Impact factor: 4.379

3.  Non-invasive human skin transcriptome analysis using mRNA in skin surface lipids.

Authors:  Takayoshi Inoue; Tetsuya Kuwano; Yuya Uehara; Michiko Yano; Naoki Oya; Naoto Takada; Shodai Tanaka; Yui Ueda; Akira Hachiya; Yoshito Takahashi; Noriyasu Ota; Takatoshi Murase
Journal:  Commun Biol       Date:  2022-03-09

4.  Non-invasive transcriptomic analysis using mRNAs in skin surface lipids obtained from children with mild-to-moderate atopic dermatitis.

Authors:  K Shima; T Inoue; Y Uehara; M Iwamura; S Fukagawa; T Kuwano; K Tanida; N Takada; M Saito-Abe; K Yamamoto-Hanada; Y Ohya; T Murase
Journal:  J Eur Acad Dermatol Venereol       Date:  2022-05-13       Impact factor: 9.228

5.  Assessing the Impact of Mechanical Damage on Full-Thickness Porcine and Human Skin Using an In Vitro Approach.

Authors:  Hinda Dabboue; Nicolas Builles; Éric Frouin; Dan Scott; Jeanne Ramos; Gilberte Marti-Mestres
Journal:  Biomed Res Int       Date:  2015-07-13       Impact factor: 3.411

6.  Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin-tape stripping technique.

Authors:  Maja-Lisa Clausen; H-C Slotved; Karen A Krogfelt; Tove Agner
Journal:  Sci Rep       Date:  2018-01-26       Impact factor: 4.379

7.  Skin Barrier Impairment due to the Occlusive Effect of Firefighter Clothing.

Authors:  Wilbert van den Eijnde; Ronald Heus; Denise Falcone; Malou Peppelman; Piet van Erp
Journal:  Ann Work Expo Health       Date:  2020-03-10       Impact factor: 2.179
  7 in total

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