Monica Mischitelli1, Anna Bellizzi2, Elena Anzivino3, Donatella Maria Rodio3, Alessandro Sciarra4, Vincenzo Gentile4, Valeria Pietropaolo5. 1. Department of Public Health and Infectious Diseases, University of Rome, Rome, Italy Department of the Science of Agriculture, Food and Environment (SAFE), University of Foggia, Foggia, Italy. 2. Department of Public Health and Infectious Diseases, Institute Pasteur, Cenci-Bolognetti Foundation, Sapienza University of Rome, Rome, Italy Department of Neuroscience, Temple University School of Medicine, Philadelphia, PA, U.S.A. 3. Department of Public Health and Infectious Diseases, University of Rome, Rome, Italy. 4. Department of Urology, "Sapienza" University of Rome, Rome, Italy. 5. Department of Public Health and Infectious Diseases, University of Rome, Rome, Italy Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology, Temple University, Philadelphia, PA, U.S.A.
Abstract
BACKGROUND: Prostate cancer (PC) is a common tumor in Western countries. Several risk factors play significant roles. MYC, BIRC5/survivin, CDC25 and P53 may contribute to PC risk. As demonstrated, human Polyomavirus BK (BKV) could affect cellular homeostasis contributing to PC pathogenesis. MATERIALS AND METHODS: Biological samples were collected from PC patients. Viral RNA was searched using quantitative polymerase chain reaction (PCR), whereas a qualitative PCR was employed to find particular viral sequences. Proper size amplicons were analyzed. Single nucleotide polymorphisms (SNPs) were detected in p53 coding regions by means of a specific PCR. C-MYC, BIRC5/survivin and CDC25 gene expression was investigated using a Retro Transcriptional Quantitative PCR. RESULTS: Viral DNA copy number was higher in cancer tissues taken from Gleason score 9 patients with Gleason score 7. Different p53 mutated compared to patients exons were found according to tumor advanced stage and a statistical significant correlation was found between Gleason score and p53 mutational rate. C-MYC, BIRC5/survivin and CDC25 expression was de-regulated according to the literature. CONCLUSION: The presence of BKV and its variants in transformed cells does not exclude viral pressure in cell immortalization. Expression of other target genes evidenced a significant change in their regulation, useful for cancer drug discovery and therapies. Copyright
BACKGROUND:Prostate cancer (PC) is a common tumor in Western countries. Several risk factors play significant roles. MYC, BIRC5/survivin, CDC25 and P53 may contribute to PC risk. As demonstrated, human Polyomavirus BK (BKV) could affect cellular homeostasis contributing to PC pathogenesis. MATERIALS AND METHODS: Biological samples were collected from PC patients. Viral RNA was searched using quantitative polymerase chain reaction (PCR), whereas a qualitative PCR was employed to find particular viral sequences. Proper size amplicons were analyzed. Single nucleotide polymorphisms (SNPs) were detected in p53 coding regions by means of a specific PCR. C-MYC, BIRC5/survivin and CDC25 gene expression was investigated using a Retro Transcriptional Quantitative PCR. RESULTS: Viral DNA copy number was higher in cancer tissues taken from Gleason score 9 patients with Gleason score 7. Different p53 mutated compared to patients exons were found according to tumor advanced stage and a statistical significant correlation was found between Gleason score and p53 mutational rate. C-MYC, BIRC5/survivin and CDC25 expression was de-regulated according to the literature. CONCLUSION: The presence of BKV and its variants in transformed cells does not exclude viral pressure in cell immortalization. Expression of other target genes evidenced a significant change in their regulation, useful for cancer drug discovery and therapies. Copyright
Authors: Elena Anzivino; Maria Antonella Zingaropoli; Marco Iannetta; Valeria Antonietta Pietropaolo; Alessandra Oliva; Francesco Iori; Antonio Ciardi; Donatella Maria Rodio; Francesca Antonini; Cesare Giovanni Fedele; Alessandra D'Abramo; Claudio Maria Mastroianni; Vincenzo Vullo; Maria Rosa Ciardi Journal: Cancer Genomics Proteomics Date: 2016 11-12 Impact factor: 4.069
Authors: Lanshan Huang; Melissa J LaBonte; Stephanie G Craig; Stephen P Finn; Emma H Allott Journal: Cancers (Basel) Date: 2022-03-08 Impact factor: 6.639