| Literature DB >> 25759468 |
Deng-Pan Lai1, Sheng Tan2, Ya-Ni Kang1, Jun Wu3, Hong-Sain Ooi4, Jian Chen5, Ting-Ting Shen5, Ying Qi5, Xiaoli Zhang5, Yan Guo1, Tao Zhu2, Bingya Liu6, Zhifeng Shao1, Xiaodong Zhao7.
Abstract
Alternative polyadenylation (APA) is an important post-transcriptional modification implicated in many diseases, including cancer. Although extensively characterized, the functional consequence of APA modulation on tumorigenesis remains elusive. Here, we developed a deep sequencing-based approach that specifically profiles 3' termini of polyadenylated RNAs (herein termed 3T-seq) and analyzed APA events in two gastric cancer cell lines and one non-transformed counterpart. Overall, we identified >28 000 poly(A) sites, 70% of which are potentially novel. Further, we observed widespread APA-mediated 3' UTR shortening of 513 genes (false discovery rate < 0.05) across gastric cancer genome. We characterized one of these genes, NET1, in detail and found that the shortening of NET1 3' UTR significantly enhances transcriptional activity. Moreover, the NET1 isoform with short 3' UTR promotes cellular migration and invasion in vitro. Collectively, our work provides an effective approach for genome-wide APA site profiling and reveals a link between APA modulation and gastric cancer metastasis.Entities:
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Year: 2015 PMID： 25759468 DOI： 10.1093/hmg/ddv089
Source DB: PubMed Journal: Hum Mol Genet ISSN： 0964-6906 Impact factor: 6.150