Harry Freitag Luglio1, Cut Gina Inggriyani2, Emy Huriyati1, Madarina Julia3, Rina Susilowati4. 1. Department of Health Nutrition, Faculty of Medicine, Universitas Gadjah Mada Indonesia. 2. Department of Anatomy Histology, Faculty of Medicine, Universitas Syiah Kuala Indonesia. 3. Department of Child Health, Faculty of Medicine, Universitas Gadjah Mada Indonesia. 4. Department of Histology and Cell Biology, Faculty of Medicine, Universitas Gadjah Mada Indonesia.
Abstract
BACKGROUND: Obesity has been linked to high dietary intake and low physical activity. Studies showed that those factors were not only regulated by environment but also by genetic. However, the relationship is less been understood in obese children and adolescents. OBJECTIVE: The objective of this study was to examine the role of SNPs in GHSR rs292216 and rs509035 on dietary intake in obese female adolescents. METHODS: This is an observational study with cross sectional design. Respondents were obese female adolescents enrolled from obesity screening done in six junior high schools in Yogyakarta. Dietary intake was measured using 6 days 24 hours inconsecutive dietary recall. Genotyping of 2 SNPs from GHSR was done using FRLP-PCR. RESULTS: There were 78 obese female adolescents joined this study. We found that no significant association between SNPs GHSR and dietary intake (p < 0.05). In addition, a SNP-SNP interaction analysis shown there is no difference between combination of GHSR rs292216 and rs509035 on dietary intake (p < 0.05). CONCLUSION: We concluded that SNPs on GHSR rs292216 and rs509035 were not related to dietary intake in Indonesian obese female adolescents. Further study is necessary to investigate the effect of those genes on dietary intake in the broader population.
BACKGROUND:Obesity has been linked to high dietary intake and low physical activity. Studies showed that those factors were not only regulated by environment but also by genetic. However, the relationship is less been understood in obesechildren and adolescents. OBJECTIVE: The objective of this study was to examine the role of SNPs in GHSRrs292216 and rs509035 on dietary intake in obese female adolescents. METHODS: This is an observational study with cross sectional design. Respondents were obese female adolescents enrolled from obesity screening done in six junior high schools in Yogyakarta. Dietary intake was measured using 6 days 24 hours inconsecutive dietary recall. Genotyping of 2 SNPs from GHSR was done using FRLP-PCR. RESULTS: There were 78 obese female adolescents joined this study. We found that no significant association between SNPs GHSR and dietary intake (p < 0.05). In addition, a SNP-SNP interaction analysis shown there is no difference between combination of GHSRrs292216 and rs509035 on dietary intake (p < 0.05). CONCLUSION: We concluded that SNPs on GHSRrs292216 and rs509035 were not related to dietary intake in Indonesian obese female adolescents. Further study is necessary to investigate the effect of those genes on dietary intake in the broader population.
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