Vasiliki Gkretsi1, Dimitrios P Bogdanos2. 1. Department of Biomedical Research and Technology, Institute for Research and Technology-Thessaly, Centre for Research and Technology-Hellas (CE.R.T.H.), Larissa, Greece vasso.gkretsi@gmail.com. 2. Department of Biomedical Research and Technology, Institute for Research and Technology-Thessaly, Centre for Research and Technology-Hellas (CE.R.T.H.), Larissa, Greece Department of Rheumatology, School of Medicine, University of Thessaly, University Hospital of Larissa, Larissa, Greece Institute of Liver Studies, King's College Hospital, London, U.K.
Abstract
BACKGROUND/AIM: Extracellular matrix (ECM) is of great significance for homeostasis in the liver. In fact, one of the stages leading to hepatocellular carcinoma (HCC) includes accumulation of excess ECM. Ras Suppressor-1 (RSU-1) is localized in the cell-ECM adhesions but its role in HCC is unexplored. MATERIALS AND METHODS: We investigated the expression and role of RSU-1 in two HCC cell lines that differ in aggressiveness; non-invasive Alexander cells and highly invasive HepG2 cells. RESULTS: Our results showed that RSU-1 expression is elevated in HepG2 cells both at the mRNA and protein level, while its silencing leads to increased cell proliferation in both cell lines. Interestingly, RSU-1 depletion from highly invasive HepG2 cells reduces cell adhesion and invasion. CONCLUSION: This is the first study to provide in vitro evidence for the involvement of RSU-1 in HCC cell invasive behavior. Copyright
BACKGROUND/AIM: Extracellular matrix (ECM) is of great significance for homeostasis in the liver. In fact, one of the stages leading to hepatocellular carcinoma (HCC) includes accumulation of excess ECM. Ras Suppressor-1 (RSU-1) is localized in the cell-ECM adhesions but its role in HCC is unexplored. MATERIALS AND METHODS: We investigated the expression and role of RSU-1 in two HCC cell lines that differ in aggressiveness; non-invasive Alexander cells and highly invasive HepG2 cells. RESULTS: Our results showed that RSU-1 expression is elevated in HepG2 cells both at the mRNA and protein level, while its silencing leads to increased cell proliferation in both cell lines. Interestingly, RSU-1 depletion from highly invasive HepG2 cells reduces cell adhesion and invasion. CONCLUSION: This is the first study to provide in vitro evidence for the involvement of RSU-1 in HCC cell invasive behavior. Copyright
Authors: Vasiliki Gkretsi; Maria Louca; Andreas Stylianou; George Minadakis; George M Spyrou; Triantafyllos Stylianopoulos Journal: Int J Mol Sci Date: 2019-01-04 Impact factor: 5.923