Terrika A Ngobili1, Hiral Shah2, Jang Pyo Park3, Kerri W Kwist3, Beau Inskeep2, Karen J L Burg4, Brian W Booth5. 1. Department of Biological Sciences, Clemson University, Clemson, SC, U.S.A. 2. Department of Bioengineering, Clemson University, Clemson, SC, U.S.A. 3. Institute for Biological Interfaces of Engineering, Clemson University, Clemson, SC, U.S.A. 4. Department of Bioengineering, Clemson University, Clemson, SC, U.S.A. Institute for Biological Interfaces of Engineering, Clemson University, Clemson, SC, U.S.A Department of Chemical Engineering, Kansas State University, Manhattan, KS, U.S.A. 5. Institute for Biological Interfaces of Engineering, Clemson University, Clemson, SC, U.S.A brbooth@clemson.edu.
Abstract
BACKGROUND: The naturally-occurring phytochemical tannic acid (TA) has anticancer properties. We have demonstrated that estrogen receptor-positive (ER+) breast cancer cells are more sensitive to effects of TA than triple-negative breast cancer cells and normal breast epithelial cells. In the present study, cells were grown on TA-crosslinked collagen beads. Growing cells remodel collagen and release TA, which affects attached cells. MATERIALS AND METHODS: The ER+ breast cancer cell line MCF7 and the normal breast epithelial cell line MCF10A were grown on TA-crosslinked collagen beads in roller bottles. Concentrations of TA in conditioned media were determined. Induced apoptosis was imaged and quantified. Caspase gene expression was calculated by real-time polymerase chain reaction (PCR). RESULTS: Both cell lines attached and grew on TA-crosslinked collagen beads where they remodeled collagen and released TA into surrounding medium. Released TA induced caspase-mediated apoptosis. CONCLUSION: TA induced apoptosis in a concentration-dependent manner, with ER+ MCF7 cells displaying more sensitivity to effects of TA. Copyright
BACKGROUND: The naturally-occurring phytochemical tannic acid (TA) has anticancer properties. We have demonstrated that estrogen receptor-positive (ER+) breast cancer cells are more sensitive to effects of TA than triple-negative breast cancer cells and normal breast epithelial cells. In the present study, cells were grown on TA-crosslinked collagen beads. Growing cells remodel collagen and release TA, which affects attached cells. MATERIALS AND METHODS: The ER+ breast cancer cell line MCF7 and the normal breast epithelial cell line MCF10A were grown on TA-crosslinked collagen beads in roller bottles. Concentrations of TA in conditioned media were determined. Induced apoptosis was imaged and quantified. Caspase gene expression was calculated by real-time polymerase chain reaction (PCR). RESULTS: Both cell lines attached and grew on TA-crosslinked collagen beads where they remodeled collagen and released TA into surrounding medium. Released TA induced caspase-mediated apoptosis. CONCLUSION:TA induced apoptosis in a concentration-dependent manner, with ER+ MCF7 cells displaying more sensitivity to effects of TA. Copyright
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