Amelie Fassbender1, Maria Debiec-Rychter2, Rieta Van Bree3, Joris Robert Vermeesch2, Christel Meuleman4, Carla Tomassetti4, Karen Peeraer4, Thomas D'Hooghe5, Dan I Lebovic6. 1. KULeuven, Department of Development and Regeneration, Organ systems, Leuven, Belgium Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium. 2. Department of Human Genetics, KU Leuven and University Hospitals Leuven, Leuven, Belgium. 3. KULeuven, Department of Development and Regeneration, Organ systems, Leuven, Belgium. 4. Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium. 5. KULeuven, Department of Development and Regeneration, Organ systems, Leuven, Belgium Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium thomas.dhooghe@uz.kuleuven.ac.be. 6. Reproductive Endocrinology & Infertility, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA lebovic@wisc.edu.
Abstract
INTRODUCTION: Fetal microchimerism has been implicated in the etiology of autoimmune diseases. This study was done to test the hypothesis that male fetal microchimerism is present in eutopic and ectopic endometrium (EM) obtained from women with endometriosis but not in eutopic EM from women without endometriosis. METHODS: A total of 31 patients were selected, including women with endometriosis (paired eutopic and ectopic EM; n = 19) and women without endometriosis (eutopic EM; n = 12). Tricolor interphase fluorescence in situ hybridization analysis was performed by cohybridization of CEP Y SpectrumAqua and CEP X SpectrumGreen (SG)/CEP Y SpectrumOrange probes. RESULTS: Ectopic EM from women with endometriosis had 75% XX chromosomes (double SG signals) and 25% X chromosomes (single SG signal). Y chromosomes were not observed in any of the eutopic/ectopic endometrial tissues from cases or controls. CONCLUSIONS: We were unable to confirm our hypothesis that male fetal microchimerism is present in eutopic and/or ectopic EM obtained from women with endometriosis.
INTRODUCTION: Fetal microchimerism has been implicated in the etiology of autoimmune diseases. This study was done to test the hypothesis that male fetal microchimerism is present in eutopic and ectopic endometrium (EM) obtained from women with endometriosis but not in eutopic EM from women without endometriosis. METHODS: A total of 31 patients were selected, including women with endometriosis (paired eutopic and ectopic EM; n = 19) and women without endometriosis (eutopic EM; n = 12). Tricolor interphase fluorescence in situ hybridization analysis was performed by cohybridization of CEP Y SpectrumAqua and CEP X SpectrumGreen (SG)/CEP Y SpectrumOrange probes. RESULTS: Ectopic EM from women with endometriosis had 75% XX chromosomes (double SG signals) and 25% X chromosomes (single SG signal). Y chromosomes were not observed in any of the eutopic/ectopic endometrial tissues from cases or controls. CONCLUSIONS: We were unable to confirm our hypothesis that male fetal microchimerism is present in eutopic and/or ectopic EM obtained from women with endometriosis.
Authors: William F N Chan; Christopher J Atkins; David Naysmith; Nicholas van der Westhuizen; Janet Woo; J Lee Nelson Journal: Arthritis Rheum Date: 2012-02
Authors: H E Viëtor; E Hallensleben; S P van Bree; E M van der Meer; S E Kaal; J Bennebroek-Gravenhorst; H H Kanhai; A Brand; F H Claas Journal: Blood Date: 2000-04-15 Impact factor: 22.113