| Literature DB >> 25749461 |
Priti Kambli1, Kanchan Ajbani1, Meeta Sadani1, Chaitali Nikam1, Anjali Shetty1, Zarir Udwadia2, Sophia B Georghiou3, Timothy C Rodwell3, Antonino Catanzaro3, Camilla Rodrigues4.
Abstract
This study correlates MICs of rifampicin (RIF) and isoniazid (INH) with GenoType MTBDRplus assay results for drug-resistant Mycobacterium tuberculosis (MTB) clinical isolates. MICs of RIF and INH were established for 84 and 90 isolates, respectively, testing 7 concentrations of each drug. Genotypic resistance to each drug was determined by GenoType MTBDRplus assay with 50 representative mutations confirmed by pyrosequencing, with mutations in the rpoB gene associated with RIF resistance and mutations in the katG and/or inhA genes associated with INH resistance. Based upon the correlation of MICs with specific genetic profiles, relative resistance levels were established for each isolate. Results indicate that MTB phenotypic resistance, currently based upon the testing of isolate susceptibility to a single drug concentration, may be more accurately profiled via quantitative MICs, and therefore, the correlation of molecular diagnostic results with specific MICs may allow for more optimal treatment of infections.Entities:
Keywords: Drug susceptibility testing; Level of resistance; Multidrug-resistant tuberculosis; Mutations
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Year: 2015 PMID： 25749461 PMCID： PMC4414878 DOI： 10.1016/j.diagmicrobio.2015.01.009
Source DB: PubMed Journal: Diagn Microbiol Infect Dis ISSN： 0732-8893 Impact factor: 2.803