Sylvaine Bastian1, Patrice Nordmann2, Elodie Creton3, Edith Malpote4, Guillaume Thiery5, Frederic Martino4, Sebastien Breurec5, Laurent Dortet6. 1. University Hospital of Pointe-à-Pitre/Abymes, Pointe-à-Pitre, Guadeloupe, France. Electronic address: sylvaine.bastian@chu-guadeloupe.fr. 2. INSERM U914 « Emerging Resistance to Antibiotic », Le Kremlin-Bicêtre, France; Associated National Reference Center to Antibiotic Resistance, Le Kremlin-Bicêtre, France; University of Fribourg, Fribourg, Switzerland; Hôpital Fribourgeois - Hôpital Cantonal, Fribourg, Switzerland. 3. INSERM U914 « Emerging Resistance to Antibiotic », Le Kremlin-Bicêtre, France; Associated National Reference Center to Antibiotic Resistance, Le Kremlin-Bicêtre, France. 4. University Hospital of Pointe-à-Pitre/Abymes, Pointe-à-Pitre, Guadeloupe, France. 5. University Hospital of Pointe-à-Pitre/Abymes, Pointe-à-Pitre, Guadeloupe, France; University of Antilles, Pointe-à-Pitre, Guadeloupe, France. 6. INSERM U914 « Emerging Resistance to Antibiotic », Le Kremlin-Bicêtre, France; Associated National Reference Center to Antibiotic Resistance, Le Kremlin-Bicêtre, France; South Paris University, Faculty of Medecine, Le Kremlin-Bicêtre, France; Assistance Publique des Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France.
Abstract
OBJECTIVES: Characterize a NDM-1 producing K. pneumoniae isolate recovered from a patient hospitalized in Guadeloupe, French West Indies, after its transfer from Cuba METHODS: Antibiotic susceptibilities were determined by the disk diffusion method, and E-test. Carbapenemase production was assessed using the Carba NP test. Antibiotic resistance determinants and their surrounding structures were characterized by PCR mapping and DNA sequencing. Transfer of the β-lactam resistance marker was attempted by liquid mating-out assays RESULTS: Here we reported the first NDM-1 producing enterobacterial isolate recovered from Caribbean islands. This K. pneumoniae isolate belongs to a new sequence type (ST1649). The blaNDM-1 gene together with the aacA4 gene were carried on a self conjugative IncR plasmid of c.a. 80kb. CONCLUSION: This study describes the first identification of a NDM-1 producer in Caribbean islands. The uncommon incompatibility group of the blaNDM-1 carrying plasmid and the uncommon ST type of the K. pneumoniae strain suggest a possible local emergence of NDM producers.
OBJECTIVES: Characterize a NDM-1 producing K. pneumoniae isolate recovered from a patient hospitalized in Guadeloupe, French West Indies, after its transfer from Cuba METHODS: Antibiotic susceptibilities were determined by the disk diffusion method, and E-test. Carbapenemase production was assessed using the Carba NP test. Antibiotic resistance determinants and their surrounding structures were characterized by PCR mapping and DNA sequencing. Transfer of the β-lactam resistance marker was attempted by liquid mating-out assays RESULTS: Here we reported the first NDM-1 producing enterobacterial isolate recovered from Caribbean islands. This K. pneumoniae isolate belongs to a new sequence type (ST1649). The blaNDM-1 gene together with the aacA4 gene were carried on a self conjugative IncR plasmid of c.a. 80kb. CONCLUSION: This study describes the first identification of a NDM-1 producer in Caribbean islands. The uncommon incompatibility group of the blaNDM-1 carrying plasmid and the uncommon ST type of the K. pneumoniae strain suggest a possible local emergence of NDM producers.
Authors: Taher Uz Zaman; Maha Alrodayyan; Maha Albladi; Mohammed Aldrees; Mohammed Ismail Siddique; Sameera Aljohani; Hanan H Balkhy Journal: BMC Infect Dis Date: 2018-05-03 Impact factor: 3.090