Rodrigo Boguen1, Favian Treulen1, Pamela Uribe1, Juana V Villegas2. 1. Centre of Reproductive Biotechnology (CEBIOR), Scientific and Technological Bioresources Nucleus (BIOREN), Temuco, Chile. 2. Centre of Reproductive Biotechnology (CEBIOR), Scientific and Technological Bioresources Nucleus (BIOREN), Temuco, Chile; Department of Internal Medicine, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile. Electronic address: juana.villegas@ufrontera.cl.
Abstract
OBJECTIVE: To determine the effect on human sperm of Escherichia coli strains separated on the basis of their ability to produce hemolysis. DESIGN: Experimental study. SETTING: University-based laboratory. PATIENT(S): Semen samples from healthy donors. INTERVENTION(S): Five million sperm, selected via the swim-up method, were incubated with 3 E. coli concentrations to obtain ratios of sperm to E. coli of 1:2, 1:16, and 1:128. The E. coli strains were: a hemolytic isolated strain (H), a nonhemolytic American Type Culture Collection strain (NH-ATCC), and a nonhemolytic isolated strain (NH-I). MAIN OUTCOME MEASURE(S): Aliquots of human sperm were used to measure progressive motility using computer-aided sperm analysis, mitochondrial membrane potential (ΔΨm) with a JC-1 (5,5',6,6' tetrachloro-1,1',3,3'-tetraethylbenzamidazolocarbocyanin iodide) and propidium iodide stain, and intracellular reactive oxygen species (iROS) with a dihydroethidium (DHE) stain. Sperm ΔΨm and iROS were measured by flow cytometry. Sperm vitality was considered the mean of propidium iodide-negative and DHE-negative cells. RESULT(S): Sperm incubated with the H strain in a 1:2 sperm to bacteria ratio demonstrated a significant decrease in motility and ΔΨm, and an increase of iROS. The NH-ATCC strain decreased sperm motility and ΔΨm, but in a ratio of sperm to bacteria of 1:128; it increased iROS at a ratio of 1:16. The NH-I strain did not affect the analyzed sperm functions, even at a 1:128 sperm to bacteria ratio. CONCLUSION(S): Results show a greater pathogenic effect on human sperm of E. coli strains with, versus without, hemolytic capacity.
OBJECTIVE: To determine the effect on human sperm of Escherichia coli strains separated on the basis of their ability to produce hemolysis. DESIGN: Experimental study. SETTING: University-based laboratory. PATIENT(S): Semen samples from healthy donors. INTERVENTION(S): Five million sperm, selected via the swim-up method, were incubated with 3 E. coli concentrations to obtain ratios of sperm to E. coli of 1:2, 1:16, and 1:128. The E. coli strains were: a hemolytic isolated strain (H), a nonhemolytic American Type Culture Collection strain (NH-ATCC), and a nonhemolytic isolated strain (NH-I). MAIN OUTCOME MEASURE(S): Aliquots of human sperm were used to measure progressive motility using computer-aided sperm analysis, mitochondrial membrane potential (ΔΨm) with a JC-1 (5,5',6,6' tetrachloro-1,1',3,3'-tetraethylbenzamidazolocarbocyanin iodide) and propidium iodide stain, and intracellular reactive oxygen species (iROS) with a dihydroethidium (DHE) stain. Sperm ΔΨm and iROS were measured by flow cytometry. Sperm vitality was considered the mean of propidium iodide-negative and DHE-negative cells. RESULT(S): Sperm incubated with the H strain in a 1:2 sperm to bacteria ratio demonstrated a significant decrease in motility and ΔΨm, and an increase of iROS. The NH-ATCC strain decreased sperm motility and ΔΨm, but in a ratio of sperm to bacteria of 1:128; it increased iROS at a ratio of 1:16. The NH-I strain did not affect the analyzed sperm functions, even at a 1:128 sperm to bacteria ratio. CONCLUSION(S): Results show a greater pathogenic effect on human sperm of E. coli strains with, versus without, hemolytic capacity.
Authors: Eva Tvrdá; Miroslava Kačániová; Andrej Baláži; Jaromír Vašíček; Jakub Vozaf; Rastislav Jurčík; Michal Ďuračka; Jana Žiarovská; Ján Kováč; Peter Chrenek Journal: Animals (Basel) Date: 2021-12-28 Impact factor: 2.752
Authors: Maria Torcia; Elisabetta Baldi; Sara Marchiani; Ilaria Baccani; Lara Tamburrino; Giorgio Mattiuz; Sabrina Nicolò; Chiara Bonaiuto; Carmen Panico; Linda Vignozzi; Alberto Antonelli; Gian Maria Rossolini Journal: Sci Rep Date: 2021-09-28 Impact factor: 4.379