Optimization of demineralization on Cyprinus carpio haematopterus scale by response surface methodology.

Fish scale, separated during fish mechanical processing, can serve as an additional source of proteins, especially of collagen proteins. To obtain high purity native collagen, it is required to carry out deproteinization and demineralization of fish scales. Therefore, the aim of this study was to determine the optimal conditions for demineralization of Cyprinus carpio haematopterus scale without loss of collagen content in HCl solution. Here, the demineralization of Cyprinus carpio haematopterus scale was optimized by response surface methodology. The optimum conditions were as follows: extraction time of 95 min, concentration of HCl of 1.0 M, and ratio of material to solution of 1:11. Under these conditions, the experimental yield of demineralization of scales was 92.7 ± 1.32 %, which was well consistent with the value predicted by the model.