Draft Genome Sequences of Acinetobacter harbinensis Strain HITLi 7T, Isolated from River Water.

Acinetobacter harbinensis HITLi strain 7(T), isolated from river water, has the ability to remove ammonium and organic chemicals at 2°C. The genome sequences might be useful for investigating the low-temperature adaptability and nitrogen or organic chemical metabolism.

GENOME ANNOUNCEMENT

Acinetobacter harbinensis HITLi strain 7T (KCTC 32411) was isolated from the Songhua River near Harbin, China. Recently, it was classified as a novel species of the genus Acinetobacter (1). A. harbinensis HITLi 7T showed ability of removing ammonium and organic chemicals at 2°C. Study on the genome sequence of A. harbinensis HITLi 7T might provide useful insight into its characteristics of cold adaptability and nitrogen or organic chemical metabolism.

The genome of A. harbinensis HITLi 7T was sequenced using the Illumina HiSeq2000 platform; 452 Mb of data were produced, and 5,421,704 reads were assembled using SOAP de novo version 2.04 (2, 3). The assembled genome has a length of 2,855,331 bp and consists of 160 contigs (N50, 41,669 bp). The overall G+C content was 41.54%. The gene prediction was performed using Glimmer version 3.02 (http://ccb.jhu.edu/software/glimmer/index.shtml) (4). The tandem repeats were predicted using the Tandem Repeats Finder (5). Functional annotation was completed by BLAST comparison to the NCBI nonredundant public database. One sRNA, 76 tRNAs, 2 copies of 5S rRNA, and 3 copies each of 16S rRNA and 23S rRNA were identified using RNAmmer (6).

The wax ester synthase/acyl-CoA:diacylglycerol acyltransferase (Wsd1) gene was present in the genome of A. harbinensis HITLi 7T, which was the important factor for cold adaptation (7). A series of autotrophic carbon dioxide fixation genes were found, such as acetyl-CoA carboxylase, pyruvate synthase, pyruvate:water dikinase, and PEP carboxylase. Among the set of genes, 183 genes were associated with xenobiotic biodegradation.

Nucleotide sequence accession numbers.

This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number JXBK00000000. The version described in this paper is version JXBK01000000.