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Literature DB >> 2574457

100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis.

D J Chin¹, R M Straubinger, S Acton, I Näthke, F M Brodsky.

Abstract

The role of the 100-kDa polypeptide components of clathrin-coated vesicles in endocytosis was investigated by microinjection of specific monoclonal antibodies. Receptor-mediated uptake of transferrin and liposomes was quantitatively inhibited. These results show that the 100-kDa polypeptides are directly involved in localized clathrin assembly at the cell periphery and are markers for the endocytic pathway. This demonstrates an in situ function of these polypeptides and the protein complexes in which they are found.

Entities: CellLine Gene

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Year: 1989 PMID： 2574457 PMCID： PMC298480 DOI： 10.1073/pnas.86.23.9289

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

25 in total

1. Pyranine as a sensitive pH probe for liposome interiors and surfaces. pH gradients across phospholipid vesicles.

Authors: K Kano; J H Fendler
Journal: Biochim Biophys Acta Date: 1978-05-18

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

3. Endocytosis of liposomes and intracellular fate of encapsulated molecules: encounter with a low pH compartment after internalization in coated vesicles.

Authors: R M Straubinger; K Hong; D S Friend; D Papahadjopoulos
Journal: Cell Date: 1983-04 Impact factor: 41.582

4. Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies.

Authors: J A Titus; R Haugland; S O Sharrow; D M Segal
Journal: J Immunol Methods Date: 1982 Impact factor: 2.303

5. Site-specific disruption of clathrin assembly produces novel structures.

Authors: G S Blank; F M Brodsky
Journal: EMBO J Date: 1986-09 Impact factor: 11.598

6. Location of the 100 kd-50 kd accessory proteins in clathrin coats.

Authors: G P Vigers; R A Crowther; B M Pearse
Journal: EMBO J Date: 1986-09 Impact factor: 11.598

7. Specificity of binding of clathrin adaptors to signals on the mannose-6-phosphate/insulin-like growth factor II receptor.

Authors: J N Glickman; E Conibear; B M Pearse
Journal: EMBO J Date: 1989-04 Impact factor: 11.598

8. Purification and properties of 100-kd proteins from coated vesicles and their reconstitution with clathrin.

Authors: B M Pearse; M S Robinson
Journal: EMBO J Date: 1984-09 Impact factor: 11.598

9. Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites.

Authors: F M Brodsky
Journal: J Cell Biol Date: 1985-12 Impact factor: 10.539

10. Immunofluorescent localization of 100K coated vesicle proteins.

Authors: M S Robinson; B M Pearse
Journal: J Cell Biol Date: 1986-01 Impact factor: 10.539

46 in total

1. The role of endocytic l1 trafficking in polarized adhesion and migration of nerve growth cones.

Authors: H Kamiguchi; F Yoshihara
Journal: J Neurosci Date: 2001-12-01 Impact factor: 6.167

2. Inhibition of the receptor-binding function of clathrin adaptor protein AP-2 by dominant-negative mutant mu2 subunit and its effects on endocytosis.

Authors: A Nesterov; R E Carter; T Sorkina; G N Gill; A Sorkin
Journal: EMBO J Date: 1999-05-04 Impact factor: 11.598

100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis.

1. Pyranine as a sensitive pH probe for liposome interiors and surfaces. pH gradients across phospholipid vesicles.

2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

3. Endocytosis of liposomes and intracellular fate of encapsulated molecules: encounter with a low pH compartment after internalization in coated vesicles.

4. Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies.

5. Site-specific disruption of clathrin assembly produces novel structures.

6. Location of the 100 kd-50 kd accessory proteins in clathrin coats.

7. Specificity of binding of clathrin adaptors to signals on the mannose-6-phosphate/insulin-like growth factor II receptor.

8. Purification and properties of 100-kd proteins from coated vesicles and their reconstitution with clathrin.

9. Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites.

10. Immunofluorescent localization of 100K coated vesicle proteins.

1. The role of endocytic l1 trafficking in polarized adhesion and migration of nerve growth cones.

2. Inhibition of the receptor-binding function of clathrin adaptor protein AP-2 by dominant-negative mutant mu2 subunit and its effects on endocytosis.

3. Cellular retention of liposome-delivered anionic compounds modulated by a probenecid-sensitive anion transporter.

4. A single common portal for clathrin-mediated endocytosis of distinct cargo governed by cargo-selective adaptors.

5. Clathrin-dependent association of CVAK104 with endosomes and the trans-Golgi network.

6. Internalization of large double-membrane intercellular vesicles by a clathrin-dependent endocytic process.

7. Dynamin-dependent transferrin receptor recycling by endosome-derived clathrin-coated vesicles.

8. The AP-2 adaptor beta2 appendage scaffolds alternate cargo endocytosis.

9. A signaling organelle containing the nerve growth factor-activated receptor tyrosine kinase, TrkA.

10. Bending a membrane: how clathrin affects budding.