Quantitative immunogold analysis reveals high glutamate levels in retinal and cortical synaptic terminals in the lateral geniculate nucleus of the macaque.

An immunogold procedure has been used on ultrathin sections of the parvo- and magnocellular layers of the dorsal lateral geniculate of the rhesus monkey to estimate quantitatively at the electron microscopic level the intensity of immunoreactivity to an antibody against glutamate over profiles of retinal, cortical, GABAergic synaptic terminals and glial cells. GABAergic terminals were identified directly by immunogold reactivity to a GABA antibody or by ultrastructural features. The results showed that in both of the main subdivisions of the geniculate the densities of immunogold particles over cortical and retinal terminals were about two- to three-fold higher than those over GABAergic terminals or glial profiles. In addition, cortical and retinal terminals showed higher positive correlations of glutamate immunogold particle densities to synaptic vesicle densities than did GABAergic terminals. These differences suggest higher and lower concentrations of glutamate corresponding to transmitter and metabolic pools of this amino acid in axon terminals of retinal and cortical origins versus GABAergic terminals, respectively, in the dorsal lateral geniculate nucleus of the macaque.