Literature DB >> 25742634

Correction: pea fiber and wheat bran fiber show distinct metabolic profiles in rats as investigated by a 1H NMR-based metabolomic approach.

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Abstract

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Year:  2015        PMID: 25742634      PMCID: PMC4351085          DOI: 10.1371/journal.pone.0119117

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


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There are errors in the Author Contributions. The correct contributions are: Conceived and designed the experiments: GL LX TF YC GJ HZ JW XC CW. Performed the experiments: GL LX TF JW. Analyzed the data: GL LX TF. Contributed reagents/materials/analysis tools: GL. Wrote the paper: GL. Additionally, there is an error in the legends for Fig. 1 and Fig. 2. “Table 1” should say “Table 5.” Please view the complete, correct Fig. 1 and Fig. 2 legends below.
Fig 1

Representative one-dimensional 1H NMR spectra urine metabolites obtained from the (A) control, (B) pea fiber, and (C) wheat bran fiber groups.

The region of δ6.2–9.5 was magnified 16 times compared with corresponding region of δ0.5–6.2 for the purpose of clarity. Metabolite keys are given in Table 5.

Fig 2

Typical 600 MHz 1H NMR spectra of plasma metabolites obtained from the (A) control, (B) pea fiber, and (C) wheat bran fiber groups.

The region of δ6.0–9.0 was magnified 24 times compared with corresponding region of δ0.5–6.0 for the purpose of clarity. Metabolite keys are given in Table 5.

Table 5

1H NMR data for metabolites in rat urine and plasma.

keysMetabolitesmoietiesδ 1H (ppm) and multiplicitysamples a
1bile acidsCH3 0.62(m), 0.75(m)U
2ButyrateCH3 0.9(t)U
3α-hydroxybutyrateCH3 0.94(t)U
4α-hydroxy-iso-valerateδCH3 0.97(d)U
5isobutyrateCH3 1.14(d)U, P
6EthanolCH3, CH2 1.19(t), 3.66(q)U, P
7methylmalonateCH3, CH1.26(d), 3.76(m)U
8α-hydroxy-n-valerateCH3, γCH2 0.89(t), 1.31(m)U
9lactateαCH, βCH3 4.13(q), 1.33(d)U, P
10alanineαCH, βCH3 3.77(q), 1.48(d)U, P
11citrullineγCH2, βCH2 1.56(m), 1.82(m)U
12acetateCH3 1.92(s)U, P
13acetamideCH3 1.99(s)U
14 N-acetylglutamateβCH2, γCH2, CH3 2.07(m), 1.88(m), 2.04(s)U
15acetoneCH3 2.25(s)U, P
16acetoacetateCH3 2.3(s)U
17succinateCH2 2.41(s)U
18α-ketoglutarateβCH2, γCH2 2.45(t), 3.01(t)U
19citrateCH2 2.55(d), 2.68(d)U, P
20methylamineCH3 2.62(s)U
21dimethylamineCH3 2.73(s)U
22trimethylamineCH3 2.88(s)U
23dimethylglycineCH3 2.93(s)U
24creatineCH3, CH2 3.04(s), 3.93(s)U, P
25creatinineCH3, CH2 3.04(s), 4.05(s)U, P
26ethanolamineCH2 3.13(t)U
27malonateCH2 3.16(s)U
28cholineOCH2, NCH2, N(CH3)3 4.07(t), 3.53(t), 3.20(s)U, P
29taurine-CH2-S, -CH2-NH2 3.26(t), 3.43(t)U
30glycineCH2 3.57(s)U
31phenylacetyglycine2,6-CH, 3,5-CH, 7-CH, 10-CH7.31(t), 7.37(m), 7.42(m), 3.68(s)U
32hippurateCH2, 3,5-CH, 4-CH, 2,6-CH3.97(d), 7.57(t), 7.65(t), 7.84(d)U
33 N-methylnicotinamideCH3, 5-CH, 4-CH, 6-CH, CH2 4.44(s), 8.18(d), 8.89(d), 8.96(d), 9.26(s)U
34β-glucose1-CH, 2-CH, 3-CH, 4-CH, 5-CH, 6-CH4.65(d), 3.25(dd), 3.49(t), 3.41(dd), 3.46(m), 3.73(dd), 3.90(dd)U, P
35α-glucose1-CH, 2-CH, 3-CH, 4-CH, 5-CH, 6-CH5.24(d), 3.54(dd), 3.71(dd), 3.42(dd), 3.84(m), 3.78(m)U, P
36allantoinCH5.40(s)U, P
37ureaNH2 5.82(s)U
38homogentisate6-CH, 5-CH6.7(d), 6.76(d),U
39 p-hydroxyphenylacetate6-CH, 2-CH, 3,5-CH3.6(s), 6.87(d), 7.15(d)U
40 m-hydroxyphenylacetate6-CH, 4-CH, 3-CH6.92(m), 7.04(d), 7.26(t)U
41nicotinate2,6-CH, 4-CH, 5-CH8.62(d), 8.25(d), 7.5(dd)U
424-aminohippurateCH2 7.71(d)U
43trigonelline2-CH, 4-CH, 6-CH, 5-CH, CH39.12(s), 8.85(m), 8.83(dd), 8.19(m), 4.44(s)U
44formateCH8.46(s)U
45unknown8.54(s)U
46HDL* CH3(CH2)n 0.84(m)P
47LDL* CH3(CH2)n 0.87(m)P
48VLDL* CH3CH2CH2C =0.89(t)P
49isoleucineαCH, βCH, βCH3, γCH2, δCH3 3.68(d), 1.99(m), 1.01(d), 1.26(m), 1.47(m), 0.94(t)P
50leucineαCH, βCH2, γCH, δCH3 3.73(t), 1.72(m), 1.72(m), 0.96(d), 0.97(d)P
51valineαCH, βCH, γCH3 3.62(d), 2.28(m), 0.99(d), 1.04(d)P
52propionateCH3, CH2 1.08(t), 2.18(q)P
533-hydroxybutyrateαCH2, βCH, γCH3 2.28(dd), 2.42(dd), 4.16(m), 1.20(d)P
54lipids (triglycerids and fatty acids)(CH2)n, CH2CH2CO, CH2C = C, CH2CO,C = CCH2C = C1.28(m),1.58(m), 2.01(m), 2.24(m), 2.76(m)P
55lysineαCH, βCH2, γCH2, εCH2 3.76(t), 1.91(m), 1.48(m), 1.72(m), 3.01(t)P
56 N-acetyl glycoproteinCH3 2.04(s)P
57 O-acetyl glycoproteinCH3 2.08(s)P
58glutamateαCH, βCH2, γCH2 3.75(m), 2.12(m), 2.35(m)P
59methionineαCH, βCH2, γCH2, S-CH3 3.87(t), 2.16(m), 2.65(t), 2.14(s)P
60pyruvateCH3 2.37(s)P
61glutamineαCH, βCH2, γCH2 3.78(m), 2.14(m), 2.45(m)P
62glycerolphosphocholineCH3, βCH2, αCH2 3.22(s), 3.69(t), 4.33(t)P
63phosphorylcholineN(CH3)3, OCH2, NCH2 3.22(s), 4.21(t), 3.61(t)P
64 myo-inositol1,3-CH, 2-CH, 5-CH, 4,6-CH3.60(dd), 4.06(t), 3.30(t), 3.63(t)P
65threonineαCH, βCH, γCH3 3.58(d), 4.24(m), 1.32(d)P
66unsaturated lipids= CH-CH2C =, -CH = CH-5.19 (m), 5.30(m)P
67tyrosine2,6-CH, 3,5-CH7.20(dd), 6.91(d)P
681-methylhistidine4-CH, 2-CH7.05(s), 7.78(s)P
69phenylalanine2,6-CH, 3,5-CH, 4-CH7.32(m), 7.42(m), 7.37(m)P
703-methylhistidine4-CH, 2-CH7.07(s), 7.67(s)P

a U, urine; P, plasma;

* HDL, high density lipoprotein; LDL, low density lipoprotein; VLDL, very low density lipoprotein; s, singlet; d, doublet; t, triplet; q, quartet; dd, doublet of doublets; m, multiplet

Representative one-dimensional 1H NMR spectra urine metabolites obtained from the (A) control, (B) pea fiber, and (C) wheat bran fiber groups.

The region of δ6.2–9.5 was magnified 16 times compared with corresponding region of δ0.5–6.2 for the purpose of clarity. Metabolite keys are given in Table 5.

Typical 600 MHz 1H NMR spectra of plasma metabolites obtained from the (A) control, (B) pea fiber, and (C) wheat bran fiber groups.

The region of δ6.0–9.0 was magnified 24 times compared with corresponding region of δ0.5–6.0 for the purpose of clarity. Metabolite keys are given in Table 5. Further, there is an error in the footnotes for Table 5. VLDL stands for “very low density lipoprotein,” not “low density lipoprotein.” Please view the complete, correct Table 5 footnotes below. a U, urine; P, plasma; * HDL, high density lipoprotein; LDL, low density lipoprotein; VLDL, very low density lipoprotein; s, singlet; d, doublet; t, triplet; q, quartet; dd, doublet of doublets; m, multiplet
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1.  Pea fiber and wheat bran fiber show distinct metabolic profiles in rats as investigated by a 1H NMR-based metabolomic approach.

Authors:  Guangmang Liu; Liang Xiao; Tingting Fang; Yimin Cai; Gang Jia; Hua Zhao; Jing Wang; Xiaoling Chen; Caimei Wu
Journal:  PLoS One       Date:  2014-12-26       Impact factor: 3.240

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