| Literature DB >> 25742290 |
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Abstract
Entities:
Year: 2015 PMID: 25742290 PMCID: PMC4351080 DOI: 10.1371/journal.pone.0119266
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 3Protein thiols, GSH and GSSG levles in bone marrow cells.
(A, B) Intracellular reduced protein thiols (A), and GSH/GSSG levels (B) in crude BMCs, Lin(−) cells and BMDDCs. Intracellular reduced thiol and GSH levels were measured by means of a sulfhydryl-specific fluorescent probe; intracellular GSSG levels were determined based on the reduction of GSSG in the presence of glutathione reductase and NADPH and on measurement of NADPH fluorescence decrease. Values are means (±SD) from at least three independent experiments, with asterisks (*) indicating statistical significant differences between (p<0.05). (C) Representative MALDI-MS images of GSH and GSSG in sectioned femur showing bone marrow distribution in WT and Gstp1/p2 mice. From left to right: scanned image of matrix sprayed MALDI slide of mouse femur with bone marrow; corresponding images of: GSH ions at m/z = 306.08 and GSSG ions at m/z = 611.14. Color heat map of the data points in the GSH and GSSG images represent averaged individual ion signal intensities of the spots.