| Literature DB >> 2574184 |
B J Potts1, M Sawyer, I C Shekarchi, T Wismer, D Huddleston.
Abstract
Contaminating bovine viral diarrhea (BVD) virus in cell cultures and in fetal bovine serum (FBS) is a well recognized problem. This study describes a direct peroxidase (DP) labeled primary antibody method for detection of pestivirus antigens in cell culture that is simple and reliable. Using this method, most bovine and porcine cell cultures, a cat lung, four mosquito and two monkey cell cultures were found contaminated with BVD virus. The rodent and human cell cultures tested were negative by this method for BVD virus.Entities:
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Year: 1989 PMID： 2574184 DOI： 10.1016/0166-0934(89)90080-3
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014