BACKGROUND AND OBJECTIVE: In regenerative medicine, there are increasing applications of low-level lasers in therapeutic protocols for treatment of diseases in soft and in bone tissues. However, there are doubts about effects on DNA, and an adequate dosimetry could improve the safety of clinical applications of these lasers. This work aimed to evaluate DNA damage in peripheral blood cells of Wistar rats induced by low-level red and infrared lasers at different fluences, powers, and emission modes according to therapeutic protocols. MATERIAL AND METHODS: Peripheral blood samples were exposed to lasers and DNA damage was accessed by comet assay. In other experiments, DNA damage was accessed in blood cells by modified comet assay using formamidopyrimidine DNA glycosylase (Fpg) and endonuclease III enzymes. RESULTS: Data show that exposure to low-level red and infrared lasers induce DNA damage depending on fluence, power and emission mode, which are targeted by Fpg and endonuclease III. CONCLUSION: Oxidative DNA damage should be considered for therapeutic efficacy and patient safety in clinical applications based on low-level red and infrared lasers.
BACKGROUND AND OBJECTIVE: In regenerative medicine, there are increasing applications of low-level lasers in therapeutic protocols for treatment of diseases in soft and in bone tissues. However, there are doubts about effects on DNA, and an adequate dosimetry could improve the safety of clinical applications of these lasers. This work aimed to evaluate DNA damage in peripheral blood cells of Wistar rats induced by low-level red and infrared lasers at different fluences, powers, and emission modes according to therapeutic protocols. MATERIAL AND METHODS: Peripheral blood samples were exposed to lasers and DNA damage was accessed by comet assay. In other experiments, DNA damage was accessed in blood cells by modified comet assay using formamidopyrimidine DNA glycosylase (Fpg) and endonuclease III enzymes. RESULTS: Data show that exposure to low-level red and infrared lasers induce DNA damage depending on fluence, power and emission mode, which are targeted by Fpg and endonuclease III. CONCLUSION: Oxidative DNA damage should be considered for therapeutic efficacy and patient safety in clinical applications based on low-level red and infrared lasers.
Authors: Larissa Alexsandra da Silva Neto Trajano; Eduardo Tavares Lima Trajano; Luiz Philippe da Silva Sergio; Adilson Fonseca Teixeira; Andre Luiz Mencalha; Ana Carolina Stumbo; Adenilson de Souza da Fonseca Journal: Lasers Med Sci Date: 2018-04-26 Impact factor: 3.161
Authors: Larissa Alexsandra da Silva Neto Trajano; Luiz Philippe da Silva Sergio; Diego Sá Leal de Oliveira; Eduardo Tavares Lima Trajano; Marco Aurélio Dos Santos Silva; Flavia de Paoli; André Luiz Mencalha; Adenilson de Souza da Fonseca Journal: Photochem Photobiol Sci Date: 2021-05-19 Impact factor: 3.982