INTRODUCTION: Rickettsiosis caused by Rickettsia rickettsii is capable of infecting vertebrates, including humans. The symptoms are high fever, headache, myalgia, nausea, vomiting, abdominal pain, and cough. Mortality can be up to 30% in untreated patients. AIMS: To prove the existence of rickettsiosis in Ensenada, Baja California, Mexico, because no human cases have been reported. MATERIAL AND METHODS: This observational, cross-sectional, descriptive study included 384 samples of humans in Ensenada, Baja California. Antibodies against R. rickettsii were measured with the kit R. rickettsii ELISA(®) Helica Biosystems, Inc., adapted for use in humans using human IgG conjugate antibodies. To determine the sensitivity and specificity, 32 human samples were submitted to IFA. Specific primers were used for the molecular diagnosis of R. rickettsii in dogs and ticks. RESULTS: The seroprevalence adjusted rickettsiosis in humans was 2.9% (95% CI: 0.8-5.3), seropositivity was not associated with sex, age, occupation, household, dogs, pet deworming program against ticks, the type of yard, and mobility of the dog between home and the street. CONCLUSIONS: With substantial agreement of k between ELISA and IFA, it follows that the results of seroprevalence of this work are reliable.
INTRODUCTION:Rickettsiosis caused by Rickettsia rickettsii is capable of infecting vertebrates, including humans. The symptoms are high fever, headache, myalgia, nausea, vomiting, abdominal pain, and cough. Mortality can be up to 30% in untreated patients. AIMS: To prove the existence of rickettsiosis in Ensenada, Baja California, Mexico, because no human cases have been reported. MATERIAL AND METHODS: This observational, cross-sectional, descriptive study included 384 samples of humans in Ensenada, Baja California. Antibodies against R. rickettsii were measured with the kit R. rickettsii ELISA(®) Helica Biosystems, Inc., adapted for use in humans using human IgG conjugate antibodies. To determine the sensitivity and specificity, 32 human samples were submitted to IFA. Specific primers were used for the molecular diagnosis of R. rickettsii in dogs and ticks. RESULTS: The seroprevalence adjusted rickettsiosis in humans was 2.9% (95% CI: 0.8-5.3), seropositivity was not associated with sex, age, occupation, household, dogs, pet deworming program against ticks, the type of yard, and mobility of the dog between home and the street. CONCLUSIONS: With substantial agreement of k between ELISA and IFA, it follows that the results of seroprevalence of this work are reliable.
Authors: Janet Foley; Luis Tinoco-Gracia; Moises Rodriguez-Lomelí; Julia Estrada-Guzmán; Maria Fierro; Elva Mattar-Lopez; Amy Peterson; Emily Pascoe; Yolanda Gonzalez; Sawako Hori-Oshima; Paige A Armstrong; Gilberto Lopez; Mariana Jacome-Ibarra; Christopher D Paddock; Oscar E Zazueta Journal: Am J Trop Med Hyg Date: 2019-07 Impact factor: 2.345
Authors: Emily G Pieracci; Juan Diego Perez De La Rosa; Daniel Luna Rubio; Mario Eduardo Solis Perales; Manuel Velasco Contreras; Naomi A Drexler; William L Nicholson; José Javier Pérez De La Rosa; Ida H Chung; Cecilia Kato; Casey Barton Behravesh; María Alejandra Gay Enríquez; Jesús Felipe González Roldan; Margarita E Villarino Journal: Zoonoses Public Health Date: 2019-08-23 Impact factor: 2.954
Authors: Gaspar Peniche-Lara; Bertha Jimenez-Delgadillo; Claudia Munoz-Zanzi; María Cárdenas-Marrufo; Carlos Pérez-Osorio; Juan Arias-León Journal: J Trop Med Date: 2018-06-05