Literature DB >> 25737991

Optimizing the Roche LightCycler(R) for Single-Tube Multiplexed RT-PCR Assays.

Clarise R Starr1, Elia T Villazana1, Richard R Chapleau1, David L Masserang1.   

Abstract

Keywords:  Duplex PCR; Duplex molecular assay; LightCycler; Real-time RT-PCR

Year:  2015        PMID： 25737991      PMCID： PMC4347082          DOI： 10.7860/JCDR/2015/9819.5361

Source DB:  PubMed          Journal:  J Clin Diagn Res        ISSN： 0973-709X


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  8 in total

1.  Identification and evaluation of new target sequences for specific detection of Bordetella pertussis by real-time PCR.

Authors:  William S Probert; Janet Ely; Kimmi Schrader; Jessica Atwell; Angela Nossoff; Stanley Kwan
Journal:  J Clin Microbiol       Date:  2008-08-27       Impact factor: 5.948

2.  Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control.

Authors:  Prithiviraj Jothikumar; Vincent Hill; Jothikumar Narayanan
Journal:  Biotechniques       Date:  2009-06       Impact factor: 1.993

3.  Optimization of the performance of the polymerase chain reaction in silicon-based microstructures.

Authors:  T B Taylor; E S Winn-Deen; E Picozza; T M Woudenberg; M Albin
Journal:  Nucleic Acids Res       Date:  1997-08-01       Impact factor: 16.971

4.  Quantification of bacterial transcripts during infection using competitive reverse transcription-PCR (RT-PCR) and LightCycler RT-PCR.

Authors:  C Goerke; M G Bayer; C Wolz
Journal:  Clin Diagn Lab Immunol       Date:  2001-03

5.  Expression of vascular endothelial growth factors A, B, C, and D and their relationships to lymph node status in lung adenocarcinoma.

Authors:  T Niki; S Iba; M Tokunou; T Yamada; Y Matsuno; S Hirohashi
Journal:  Clin Cancer Res       Date:  2000-06       Impact factor: 12.531

6.  Polyphenols downregulate PAI-1 gene expression in cultured human coronary artery endothelial cells: molecular contributor to cardiovascular protection.

Authors:  Consuelo Pasten; Nelida C Olave; Lihua Zhou; Edlue M Tabengwa; Paul E Wolkowicz; Hernan E Grenett
Journal:  Thromb Res       Date:  2007-03-26       Impact factor: 3.944

7.  Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification.

Authors:  A Akane; K Matsubara; H Nakamura; S Takahashi; K Kimura
Journal:  J Forensic Sci       Date:  1994-03       Impact factor: 1.832

8.  Quantitative determination, by real-time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast.

Authors:  Zhenhuan Zhang; Hiroko Yamashita; Tatsuya Toyama; Yoko Omoto; Hiroshi Sugiura; Yasuo Hara; Xueqing Wu; Shunzo Kobayashi; Hirotaka Iwase
Journal:  Breast Cancer Res       Date:  2003-10-09       Impact factor: 6.466
  8 in total

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