Stig Molsted1, Jesper Løvind Andersen2, Adrian Paul Harrison3, Inge Eidemak4, Abigail Louise Mackey2. 1. Department of Cardiology, Nephrology & Endocrinology, Nordsjaellands Hospital, Dyrehavevej 29, 3400 Hillerød, University of Copenhagen, Copenhagen, Denmark. 2. Institute of Sports Medicine Copenhagen, Department of Orthopaedic Surgery M, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark. 3. Department of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, Copenhagen University, Copenhagen, Denmark. 4. Department of Nephrology P, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark.
Abstract
INTRODUCTION: The aim of this study was to assess the effect of high-intensity resistance training on satellite cell (SC) and myonuclear number in the muscle of patients undergoing dialysis. METHODS: Patients (n = 21) underwent a 16-week control period, followed by 16 weeks of resistance training 3 times weekly. SC and myonuclear number were determined by immunohistochemistry of vastus lateralis muscle biopsy cross-sections. Knee extension torque was tested in a dynamometer. RESULTS: During training, SCs/type I fibers increased by 15%, whereas SCs/type II fibers remained unchanged. Myonuclear content of type II, but not type I, fibers increased with training. Before the control period, the SC content of type II fibers was lower than that of type I fibers, whereas contents were comparable when normalized to fiber area. Torque increased after training. CONCLUSIONS: Increased myonuclear content of type II muscle fibers of dialysis patients who perform resistance training suggests that SC dysfunction is not the limiting factor for muscle growth.
INTRODUCTION: The aim of this study was to assess the effect of high-intensity resistance training on satellite cell (SC) and myonuclear number in the muscle of patients undergoing dialysis. METHODS:Patients (n = 21) underwent a 16-week control period, followed by 16 weeks of resistance training 3 times weekly. SC and myonuclear number were determined by immunohistochemistry of vastus lateralis muscle biopsy cross-sections. Knee extension torque was tested in a dynamometer. RESULTS: During training, SCs/type I fibers increased by 15%, whereas SCs/type II fibers remained unchanged. Myonuclear content of type II, but not type I, fibers increased with training. Before the control period, the SC content of type II fibers was lower than that of type I fibers, whereas contents were comparable when normalized to fiber area. Torque increased after training. CONCLUSIONS: Increased myonuclear content of type II muscle fibers of dialysis patients who perform resistance training suggests that SC dysfunction is not the limiting factor for muscle growth.
Authors: Jonathan B Muyskens; Douglas M Foote; Nathan J Bigot; Lisa A Strycker; Keith Smolkowski; Tessa K Kirkpatrick; Brick A Lantz; Steven N Shah; Craig G Mohler; Brian A Jewett; Erin C Owen; Hans C Dreyer Journal: J Appl Physiol (1985) Date: 2019-07-25
Authors: Camille R Brightwell; Ameya S Kulkarni; William Paredes; Kehao Zhang; Jaclyn B Perkins; Knubian J Gatlin; Matthew Custodio; Hina Farooq; Bushra Zaidi; Rima Pai; Rupinder S Buttar; Yan Tang; Michal L Melamed; Thomas H Hostetter; Jeffrey E Pessin; Meredith Hawkins; Christopher S Fry; Matthew K Abramowitz Journal: JCI Insight Date: 2021-12-22