Amir Reza Tafreshian1, Mehdi Etemadi2, Reza Farid-Hosseini3, Mansoor Salhi4, Jalil Tavakkol Afshari1. 1. Immunology Research Group, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran. 2. Neurology Department and HTLVI Foundation, Ghaem Medical Center, Mashhad University of Medical Sciences, Mashhad, Iran. 3. Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 4. Genetics Department School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Abstract
OBJECTIVES: HTLVI-1 is the first human retrovirus with limited endemic regions in the world. The epidemiological studies have shown that the genetic background and immune response to the virus have a significant role in HTLV-I-associated diseases. Among the genes are involved in HTLV-I infection, the role of human leukocytes antigen (HLA) have been studied in different population. In the present study we examined the association between HLA-DQB1 alleles and HTLV-I infection in HAM/TSP patients, HTLV-I carriers and healthy controls in north east of Iran, Mashhad. MATERIALS AND METHODS: The blood samples of 16 patients with HAM/TSP, 20 HTLV-1 carriers, and 30 healthy individuals were taken and DNA was extracted by salting out method. HLA-DQB1 typing was performed using PCR-SSP method and the frequency of HLA-DQB1 alleles were compared by Fischer Exact Test. RESULTS: There was a significant difference between HAM/TSP patients and healthy controls in the frequency of HLA-DQB1*07 (P=0.004, RR=7). Furthermore, we found that possession of HLA- DQB1*02 or HLA-DQB1*05 increased the risk of disease 1.5 times. CONCLUSION: The data presented here suggest that both HLA-DQB1*07 and HLA-DQB1*06 are associated with disease development.
OBJECTIVES: HTLVI-1 is the first human retrovirus with limited endemic regions in the world. The epidemiological studies have shown that the genetic background and immune response to the virus have a significant role in HTLV-I-associated diseases. Among the genes are involved in HTLV-I infection, the role of human leukocytes antigen (HLA) have been studied in different population. In the present study we examined the association between HLA-DQB1 alleles and HTLV-I infection in HAM/TSPpatients, HTLV-I carriers and healthy controls in north east of Iran, Mashhad. MATERIALS AND METHODS: The blood samples of 16 patients with HAM/TSP, 20 HTLV-1 carriers, and 30 healthy individuals were taken and DNA was extracted by salting out method. HLA-DQB1 typing was performed using PCR-SSP method and the frequency of HLA-DQB1 alleles were compared by Fischer Exact Test. RESULTS: There was a significant difference between HAM/TSPpatients and healthy controls in the frequency of HLA-DQB1*07 (P=0.004, RR=7). Furthermore, we found that possession of HLA- DQB1*02 or HLA-DQB1*05 increased the risk of disease 1.5 times. CONCLUSION: The data presented here suggest that both HLA-DQB1*07 and HLA-DQB1*06 are associated with disease development.
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