D Rezazadeh1,2, M-T Moradi3, A Kazemi4, K Mansouri1,2. 1. Department of Molecular Medicine, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran. 2. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran. 3. Medical Biology Research Center and Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran. 4. Department of Laboratory Hematology, School of Para Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.
Abstract
INTRODUCTION: Acute lymphoblastic leukemia (ALL) is the most prevalent malignancy among children and makes up 23% of total childhood cancers worldwide. Pre-B ALL is one of the most common ALLs, comprising about 80% of childhood cases. A variety of genes are involved in metabolizing carcinogens. These gene polymorphisms can result in less efficient or overly-down metabolic pathways, which may contribute to the susceptibility to develop cancer. Glutathione S-transferase omega (GSTO) is a new known class among GSTs superfamily. GSTO1 and GSTO2 polymorphisms have been reported to be related to several types of disease. We assessed the association between GSTO1 and GSTO2 polymorphisms and childhood pre-B ALL risk in Iran. METHODS: This case-control study analyzed GSTO1 A140D (rs. 4925) and GSTO2 N142D (rs. 156697) gene polymorphisms using a polymerase chain reaction-restriction fragment length polymorphism method, in 100 patients and 120 healthy controls. RESULTS: The genotype frequencies were not significantly different between patients and healthy controls. Odds ratio (95% confidence intervals) for mutant homozygotes were 1.54 (0.628-3.778) and 0.791 (0.349-1.793) for GSTO1 A140D and GSTO2 N142D, respectively. CONCLUSION: This study found no significant association between Pre-B ALL and GSTO1 A140D and GSTO2 N142D polymorphisms.
INTRODUCTION:Acute lymphoblastic leukemia (ALL) is the most prevalent malignancy among children and makes up 23% of total childhood cancers worldwide. Pre-B ALL is one of the most common ALLs, comprising about 80% of childhood cases. A variety of genes are involved in metabolizing carcinogens. These gene polymorphisms can result in less efficient or overly-down metabolic pathways, which may contribute to the susceptibility to develop cancer. Glutathione S-transferase omega (GSTO) is a new known class among GSTs superfamily. GSTO1 and GSTO2 polymorphisms have been reported to be related to several types of disease. We assessed the association between GSTO1 and GSTO2 polymorphisms and childhood pre-B ALL risk in Iran. METHODS: This case-control study analyzed GSTO1A140D (rs. 4925) and GSTO2N142D (rs. 156697) gene polymorphisms using a polymerase chain reaction-restriction fragment length polymorphism method, in 100 patients and 120 healthy controls. RESULTS: The genotype frequencies were not significantly different between patients and healthy controls. Odds ratio (95% confidence intervals) for mutant homozygotes were 1.54 (0.628-3.778) and 0.791 (0.349-1.793) for GSTO1A140D and GSTO2N142D, respectively. CONCLUSION: This study found no significant association between Pre-B ALL and GSTO1A140D and GSTO2N142D polymorphisms.
Authors: Shahid M Baba; Arshad A Pandith; Zafar A Shah; Sajad A Geelani; Javid R Bhat; Ayaz Gul; Sameer A Guru; Hamed A El-Serehy; Abid M Koul; Sheikh Mansoor Journal: Front Oncol Date: 2021-10-14 Impact factor: 6.244