Yunmee Lho1, Carel W le Roux2, Hyeon Soo Park3, Gon Sup Kim3, Jeeyoun Jung4, Geum-Sook Hwang5, Youn Kyoung Seo6, Tae Kyung Ha7, Eunyoung Ha8. 1. Department of Biochemistry, Pain Research Center, School of Medicine, Keimyung University, Daegu, Republic of Korea. 2. Diabetes Complications Research Center, UCD Conway Institute, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland. 3. Research Institute of Life Science, College of Veterinary Medicine (BK21 plus project), Kyeongsang National University, Jinju, Republic of Korea. 4. KM Health Technology Research Group, Medical Research Division, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea. 5. Integrated Metabolomics Research Group of Seoul Center, Korea Basic Science Institute, Seoul, Republic of Korea. 6. Department of Anatomy and Cell Biology, College of Medicine, Hanyang University, Seoul, Republic of Korea. 7. Department of Surgery, College of Medicine, Hanyang University, Seoul, Republic of Korea. htksurgeon@gmail.com. 8. Department of Biochemistry, Pain Research Center, School of Medicine, Keimyung University, Daegu, Republic of Korea. hanne.md@gmail.com.
Abstract
BACKGROUND: We evaluated metabolic changes after vertical sleeve gastrectomy (VSG) surgery in a rat model using proteomics and metabolomic profiling in liver and serum. METHODS: Rats were randomly divided into two groups: sham (n = 10) and VSG (n = 12). Food intake, body weight, blood glucose, insulin, and thyroid hormone levels were measured. Two-dimensional electrophoresis, nuclear resonance spectroscopy, mass spectroscopy, immunofluorescence, and immunoblot analyses were used to determine and validate changes in metabolites and proteins in liver tissue and serum samples. RESULTS: Food intake and body weight decreased after VSG group (p < 0.05 and p < 0.05, respectively). Random blood glucose (sham, 183.3 ± 5.6 mg/dL; VSG, 138.5 ± 3.7 mg/dL) decreased while random insulin (sham, 0.45 ± 0.16 μg/L; VSG, 1.05 ± 0.18 μg/L) increased after VSG (p < 0.05 and p < 0.01, respectively). We found that expressions of gluconeogenic enzymes (phosphoenolpyruvate carboxykinase-1 and glucose-6-phosphatase) and concentrations of pyruvate and malate decreased while lactate, NADH, NADPH, glucose, and AMP/ATP ratio increased after VSG. Thyroid hormones, triiodothyronine (T3) and free thyroxine (fT4), decreased after VSG. CONCLUSION: This study proves that VSG suppresses hepatic glucose production.
BACKGROUND: We evaluated metabolic changes after vertical sleeve gastrectomy (VSG) surgery in a rat model using proteomics and metabolomic profiling in liver and serum. METHODS:Rats were randomly divided into two groups: sham (n = 10) and VSG (n = 12). Food intake, body weight, blood glucose, insulin, and thyroid hormone levels were measured. Two-dimensional electrophoresis, nuclear resonance spectroscopy, mass spectroscopy, immunofluorescence, and immunoblot analyses were used to determine and validate changes in metabolites and proteins in liver tissue and serum samples. RESULTS: Food intake and body weight decreased after VSG group (p < 0.05 and p < 0.05, respectively). Random blood glucose (sham, 183.3 ± 5.6 mg/dL; VSG, 138.5 ± 3.7 mg/dL) decreased while random insulin (sham, 0.45 ± 0.16 μg/L; VSG, 1.05 ± 0.18 μg/L) increased after VSG (p < 0.05 and p < 0.01, respectively). We found that expressions of gluconeogenic enzymes (phosphoenolpyruvate carboxykinase-1 and glucose-6-phosphatase) and concentrations of pyruvate and malate decreased while lactate, NADH, NADPH, glucose, and AMP/ATP ratio increased after VSG. Thyroid hormones, triiodothyronine (T3) and free thyroxine (fT4), decreased after VSG. CONCLUSION: This study proves that VSG suppresses hepatic glucose production.
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