Fergal J Duffy1, Pauline M Rudd1. 1. National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Co. Dublin, Ireland.
Abstract
MOTIVATION: Sequencing glycan structures is a difficult problem that requires the use of multiple experimental approaches. One powerful approach to glycan sequencing is the combination of liquid chromatography with sequential exoglycosidase digestions; however, interpreting this can be difficult and time-consuming. To aid this process, we introduce GlycoProfileAssigner, software for automated structural assignment of glycan profile data from liquid chromatography experiments. RESULTS: GlycoProfileAssigner has been tested on human IgG data, and can retrieve the correct structure in 14 out of 16 peaks tested. AVAILABILITY AND IMPLEMENTATION: The programme and its source code is available at https://bitbucket.org/fergaljd/glycoprofileassigner CONTACT: : pauline.rudd@nibrt.ie SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
MOTIVATION: Sequencing glycan structures is a difficult problem that requires the use of multiple experimental approaches. One powerful approach to glycan sequencing is the combination of liquid chromatography with sequential exoglycosidase digestions; however, interpreting this can be difficult and time-consuming. To aid this process, we introduce GlycoProfileAssigner, software for automated structural assignment of glycan profile data from liquid chromatography experiments. RESULTS: GlycoProfileAssigner has been tested on human IgG data, and can retrieve the correct structure in 14 out of 16 peaks tested. AVAILABILITY AND IMPLEMENTATION: The programme and its source code is available at https://bitbucket.org/fergaljd/glycoprofileassigner CONTACT: : pauline.rudd@nibrt.ie SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
Authors: Ian Walsh; Terry Nguyen-Khuong; Katherine Wongtrakul-Kish; Shi Jie Tay; Daniel Chew; Tasha José; Christopher H Taron; Pauline M Rudd Journal: Bioinformatics Date: 2019-02-15 Impact factor: 6.937