The aim is to develop irradiated nanodiamonds (INDs) as a molecularly targeted contrast agent for high-resolution and phenotype-specific detection of breast cancer with photoacoustic (PA) imaging. The surface of acid treated radiation-damaged nanodiamonds was grafted with PEG to improve its stability and circulation time in blood, followed by conjugation to an anti-HER2 peptide with a final nanoparticle size of approximately 92 nm. Immunocompetent mice bearing orthotopic HER2-positive or negative tumors were administered INDs and PA imaged using an 820-nm near-infrared laser. PA images demonstrated that INDs accumulate in tumors and completely delineated the entire tumor within 10 h. HER2 targeting significantly enhanced imaging of HER2-positive tumors. Pathological examination demonstrated INDs are nontoxic. PA technology is adaptable to low-cost bedside medicine, and with new contrast agents described herein, PA can achieve high-resolution (sub-mm) and phenotype-specific monitoring of cancer growth.
The aim is to develop irradiated nanopan class="Chemical">diamonds (INDs) as a molecularly targeted contrast agent for high-resolution and phenotype-specific detection of breast cancer with photoacoustic (PA) imaging. The surface of acid treated radiation-damaged nanodiamonds was grafted with PEG to improve its stability and circulation time in blood, followed by conjugation to an anti-HER2 peptide with a final nanoparticle size of approximately 92 nm. Immunocompetent mice bearing orthotopic HER2-positive or negative tumors were administered INDs and PA imaged using an 820-nm near-infrared laser. PA images demonstrated that INDs accumulate in tumors and completely delineated the entire tumor within 10 h. HER2 targeting significantly enhanced imaging of HER2-positive tumors. Pathological examination demonstrated INDs are nontoxic. PA technology is adaptable to low-cost bedside medicine, and with new contrast agents described herein, PA can achieve high-resolution (sub-mm) and phenotype-specific monitoring of cancer growth.
Entities:
Keywords:
HER2; breast cancer; contrast agent; nanodiamond; photoacoustic imaging
Authors: Charles L Vogel; Melody A Cobleigh; Debu Tripathy; John C Gutheil; Lyndsay N Harris; Louis Fehrenbacher; Dennis J Slamon; Maureen Murphy; William F Novotny; Michael Burchmore; Steven Shak; Stanford J Stewart; Michael Press Journal: J Clin Oncol Date: 2002-02-01 Impact factor: 44.544
Authors: Grigory S Filonov; Arie Krumholz; Jun Xia; Junjie Yao; Lihong V Wang; Vladislav V Verkhusha Journal: Angew Chem Int Ed Engl Date: 2011-12-23 Impact factor: 15.336
Authors: Jasmine M Tam; Justina O Tam; Avinash Murthy; Davis R Ingram; Li Leo Ma; Kort Travis; Keith P Johnston; Konstantin V Sokolov Journal: ACS Nano Date: 2010-04-27 Impact factor: 15.881
Authors: Aishik Chakraborty; Nicolas J Mucci; Ming Li Tan; Ashleigh Steckley; Ti Zhang; M Laird Forrest; Prajnaparamita Dhar Journal: Langmuir Date: 2015-04-28 Impact factor: 3.882
Authors: Hong Zhang; Hongxiu Diao; Lixin Jia; Yujing Yuan; Douglas H Thamm; Huanan Wang; Yipeng Jin; Shimin Pei; Bin Zhou; Fang Yu; Linna Zhao; Nan Cheng; Hongchao Du; Ying Huang; Di Zhang; Degui Lin Journal: PLoS One Date: 2017-12-05 Impact factor: 3.240
Authors: Mitchell J Duffy; Oriol Planas; Andreas Faust; Thomas Vogl; Sven Hermann; Michael Schäfers; Santi Nonell; Cristian A Strassert Journal: Photoacoustics Date: 2018-01-11