Patricia Aguilar-Calvo1, Christine Fast2, Kerstin Tauscher2, Juan-Carlos Espinosa1, Martin H Groschup2, Muhammad Nadeem3, Wilfred Goldmann4, Jan Langeveld5, Alex Bossers5, Olivier Andreoletti6, Juan-María Torres1. 1. Centro de Investigación en Sanidad Animal, Valdeolmos, Madrid, Spain. 2. Institute for Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Südufer, Greifswald-InselRiems. 3. Department of Pathology, University of Veterinary Medicine Hannover, Germany. 4. The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Midlothian, United Kingdom. 5. Central Veterinary Institute, Wageningen UR, Lelystad, The Netherlands. 6. UMR INRA-ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, France.
Abstract
BACKGROUND: The prion protein-encoding gene (PRNP) is one of the major determinants for scrapie occurrence in sheep and goats. However, its effect on bovine spongiform encephalopathy (BSE) transmission to goats is not clear. METHODS: Goats harboring wild-type, R/Q211 or Q/K222 PRNP genotypes were orally inoculated with a goat-BSE isolate to assess their relative susceptibility to BSE infection. Goats were killed at different time points during the incubation period and after the onset of clinical signs, and their brains as well as several peripheral tissues were analyzed for the accumulation of pathological prion protein (PrP(Sc)) and prion infectivity by mouse bioassay. RESULTS: R/Q211 goats displayed delayed clinical signs compared with wild-type goats. Deposits of PrP(Sc) were detected only in brain, whereas infectivity was present in peripheral tissues too. In contrast, none of the Q/K222 goats showed any evidence of clinical prion disease. No PrP(Sc) accumulation was observed in their brains or peripheral tissues, but very low infectivity was detected in some tissues very long after inoculation (44-45 months). CONCLUSIONS: These results demonstrate that transmission of goat BSE is genotype dependent, and they highlight the pivotal protective effect of the K222 PRNP variant in the oral susceptibility of goats to BSE.
BACKGROUND: The prion protein-encoding gene (PRNP) is one of the major determinants for scrapie occurrence in sheep and goats. However, its effect on bovine spongiform encephalopathy (BSE) transmission to goats is not clear. METHODS: Goats harboring wild-type, R/Q211 or Q/K222 PRNP genotypes were orally inoculated with a goat-BSE isolate to assess their relative susceptibility to BSE infection. Goats were killed at different time points during the incubation period and after the onset of clinical signs, and their brains as well as several peripheral tissues were analyzed for the accumulation of pathological prion protein (PrP(Sc)) and prion infectivity by mouse bioassay. RESULTS: R/Q211 goats displayed delayed clinical signs compared with wild-type goats. Deposits of PrP(Sc) were detected only in brain, whereas infectivity was present in peripheral tissues too. In contrast, none of the Q/K222 goats showed any evidence of clinical prion disease. No PrP(Sc) accumulation was observed in their brains or peripheral tissues, but very low infectivity was detected in some tissues very long after inoculation (44-45 months). CONCLUSIONS: These results demonstrate that transmission of goat BSE is genotype dependent, and they highlight the pivotal protective effect of the K222 PRNP variant in the oral susceptibility of goats to BSE.
Authors: Maria Vitale; Sergio Migliore; Maria La Giglia; Placido Alberti; Vincenzo Di Marco Lo Presti; Jan P M Langeveld Journal: BMC Vet Res Date: 2016-07-15 Impact factor: 2.741
Authors: W Goldmann; E Marier; P Stewart; T Konold; S Street; J Langeveld; O Windl; A Ortiz-Pelaez Journal: Vet Rec Date: 2016-01-11 Impact factor: 2.695
Authors: C Fast; W Goldmann; P Berthon; K Tauscher; O Andréoletti; I Lantier; C Rossignol; A Bossers; J G Jacobs; N Hunter; M H Groschup; F Lantier; J P M Langeveld Journal: Vet Res Date: 2017-09-19 Impact factor: 3.683
Authors: Alba Marín-Moreno; Patricia Aguilar-Calvo; Mohammed Moudjou; Juan Carlos Espinosa; Vincent Béringue; Juan María Torres Journal: Sci Rep Date: 2019-08-06 Impact factor: 4.379