PURPOSE: To study the responses of the general population of retinal ganglion cells (Brn3a(+)RGCs) versus the intrinsically photosensitive RGCs (melanopsin-expressing RGCs [m(+)RGCs]) to ocular hypertension (OHT), the effects of brain-derived neurotrophic factor (BDNF) on the survival of axonally intact and axonally nonintact RGCs, and the correlation of vascular integrity with sectorial RGC loss. METHODS: In Sprague-Dawley rats, 5 μg BDNF or vehicle was intravitreally injected into the left eye followed by laser photocoagulation of the limbal tissues. To identify RGCs with an active retrograde axonal transport, Fluorogold was applied to both superior colliculi 1 week before euthanasia (FG(+)RGCs). Retinas were dissected 12 or 15 days after lasering and immunoreacted against Brn3a (to identify all RGCs except m(+)RGCs), melanopsin, or RECA1 (inner retinal vasculature). RESULTS: Ocular hypertension resulted at 12 to 15 days in sectorial loss of FG(+)RGCs (78%-84%, respectively) while Brn3a(+)RGCs were significantly greater, indicating that a substantial proportion (approximately 21%-26%) of RGCs with their retrograde axonal transport impaired survive in the retina. Brain-derived neurotrophic factor increased the survival of Brn3a(+)RGCs to 81% to 67% at 12 to 15 days, respectively. The inner retinal vasculature showed no abnormalities that could account for the sectorial loss of RGCs. At 12 to 15 days, m(+)RGCs decreased to approximately 50% to 51%, but this loss was diffuse across the retina and was not prevented by BDNF. CONCLUSIONS: The responses of m(+)RGCs against OHT-induced retinal degeneration and neuroprotection differ from those of Brn3a(+)RGCs; while OHT induces similar loss of Brn3a(+)RGCs and m(+)RGCs, Brn3a(+)RGCs are lost in sectors and can be rescued with BDNF, but m(+)RGCs do not respond to BDNF and their loss is diffuse. Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.
PURPOSE: To study the responses of the general population of retinal ganglion cells (Brn3a(+)RGCs) versus the intrinsically photosensitive RGCs (melanopsin-expressing RGCs [m(+)RGCs]) to ocular hypertension (OHT), the effects of brain-derived neurotrophic factor (BDNF) on the survival of axonally intact and axonally nonintact RGCs, and the correlation of vascular integrity with sectorial RGC loss. METHODS: In Sprague-Dawley rats, 5 μg BDNF or vehicle was intravitreally injected into the left eye followed by laser photocoagulation of the limbal tissues. To identify RGCs with an active retrograde axonal transport, Fluorogold was applied to both superior colliculi 1 week before euthanasia (FG(+)RGCs). Retinas were dissected 12 or 15 days after lasering and immunoreacted against Brn3a (to identify all RGCs except m(+)RGCs), melanopsin, or RECA1 (inner retinal vasculature). RESULTS:Ocular hypertension resulted at 12 to 15 days in sectorial loss of FG(+)RGCs (78%-84%, respectively) while Brn3a(+)RGCs were significantly greater, indicating that a substantial proportion (approximately 21%-26%) of RGCs with their retrograde axonal transport impaired survive in the retina. Brain-derived neurotrophic factor increased the survival of Brn3a(+)RGCs to 81% to 67% at 12 to 15 days, respectively. The inner retinal vasculature showed no abnormalities that could account for the sectorial loss of RGCs. At 12 to 15 days, m(+)RGCs decreased to approximately 50% to 51%, but this loss was diffuse across the retina and was not prevented by BDNF. CONCLUSIONS: The responses of m(+)RGCs against OHT-induced retinal degeneration and neuroprotection differ from those of Brn3a(+)RGCs; while OHT induces similar loss of Brn3a(+)RGCs and m(+)RGCs, Brn3a(+)RGCs are lost in sectors and can be rescued with BDNF, but m(+)RGCs do not respond to BDNF and their loss is diffuse. Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.
Authors: María F González Fleitas; Julián Devouassoux; Marcos L Aranda; Hernán H Dieguez; Juan S Calanni; Agustina Iaquinandi; Pablo H Sande; Damián Dorfman; Ruth E Rosenstein Journal: Mol Neurobiol Date: 2021-03-31 Impact factor: 5.590
Authors: José A Fernández-Albarral; Juan J Salazar; Rosa de Hoz; Eva M Marco; Beatriz Martín-Sánchez; Elena Flores-Salguero; Elena Salobrar-García; Inés López-Cuenca; Vicente Barrios-Sabador; Marcelino Avilés-Trigueros; Francisco J Valiente-Soriano; Juan A Miralles de Imperial-Ollero; Manuel Vidal-Sanz; Alberto Triviño; José M Ramírez; Meritxell López-Gallardo; Ana I Ramírez Journal: Int J Mol Sci Date: 2021-02-19 Impact factor: 5.923
Authors: Manuel Vidal-Sanz; Francisco M Nadal-Nicolás; Francisco J Valiente-Soriano; Marta Agudo-Barriuso; Maria P Villegas-Pérez Journal: Neural Regen Res Date: 2015-08 Impact factor: 5.135
Authors: Eline Dekeyster; Emiel Geeraerts; Tom Buyens; Chris Van den Haute; Veerle Baekelandt; Lies De Groef; Manuel Salinas-Navarro; Lieve Moons Journal: PLoS One Date: 2015-11-11 Impact factor: 3.240
Authors: Francisco M Nadal-Nicolás; Caridad Galindo-Romero; Francisco J Valiente-Soriano; María Barberà-Cremades; Carlos deTorre-Minguela; Manuel Salinas-Navarro; Pablo Pelegrín; Marta Agudo-Barriuso Journal: Sci Rep Date: 2016-12-08 Impact factor: 4.379