Shu-Wen Hsuan1, Charng-Cherng Chyau2, Hsiao-Yu Hung1, Jing-Hsien Chen3,4, Fen-Pi Chou5,6,7. 1. Institute of Biochemistry and Biotechnology, Medical College, Chung Shan Medical University, Taichung, Taiwan. 2. Research Institute of Biotechnology, Hung Kuang University, Taichung, Taiwan. 3. School of Nutrition, Chung Shan Medical University, Taichung, Taiwan. cjh0828@csmu.edu.tw. 4. Chung Shan Medical University, No. 110, Sec. 1, Jiangou N. Rd., South Dist., Taichung City, 402, Taiwan. cjh0828@csmu.edu.tw. 5. Institute of Biochemistry and Biotechnology, Medical College, Chung Shan Medical University, Taichung, Taiwan. fpchou@csmu.edu.tw. 6. Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan. fpchou@csmu.edu.tw. 7. Chung Shan Medical University, No. 110, Sec. 1, Jiangou N. Rd., South Dist., Taichung City, 402, Taiwan. fpchou@csmu.edu.tw.
Abstract
PURPOSE: Wasabia japonica (wasabi) has been shown to exhibit properties of detoxification, anti-inflammation and the induction of apoptosis in cancer cells. This study aimed to investigate the molecular mechanism of the cytotoxicity of wasabi extract (WE) in colon cancer cells to evaluate the potential of wasabi as a functional food for chemoprevention. METHODS: Colo 205 cells were treated with different doses of WE, and the cytotoxicity was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide. Apoptosis and autophagy were detected by 4',6-diamidino-2-phenylindole, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbo-yanine iodide and staining for acidic vascular organelles (AVOs), along with Western blotting. RESULTS: The results demonstrated that WE induced the extrinsic pathway and mitochondrial death machinery through the activation of TNF-α, Fas-L, caspases, truncated Bid and cytochrome C. WE also induced autophagy by decreasing the phosphorylation of Akt and mTOR and promoting the expression of microtubule-associated protein 1 light chain 3-II and AVO formation. An in vivo xenograft model verified that tumor growth was delayed by WE treatment. CONCLUSION: Our studies revealed that WE exhibits anti-colon cancer properties through the induction of apoptosis and autophagy. These results provide support for the application of WE as a chemopreventive functional food and as a prospective treatment of colon cancer.
PURPOSE:Wasabia japonica (wasabi) has been shown to exhibit properties of detoxification, anti-inflammation and the induction of apoptosis in cancer cells. This study aimed to investigate the molecular mechanism of the cytotoxicity of wasabi extract (WE) in colon cancer cells to evaluate the potential of wasabi as a functional food for chemoprevention. METHODS:Colo 205 cells were treated with different doses of WE, and the cytotoxicity was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide. Apoptosis and autophagy were detected by 4',6-diamidino-2-phenylindole, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbo-yanine iodide and staining for acidic vascular organelles (AVOs), along with Western blotting. RESULTS: The results demonstrated that WE induced the extrinsic pathway and mitochondrial death machinery through the activation of TNF-α, Fas-L, caspases, truncated Bid and cytochrome C. WE also induced autophagy by decreasing the phosphorylation of Akt and mTOR and promoting the expression of microtubule-associated protein 1 light chain 3-II and AVO formation. An in vivo xenograft model verified that tumor growth was delayed by WE treatment. CONCLUSION: Our studies revealed that WE exhibits anti-colon cancer properties through the induction of apoptosis and autophagy. These results provide support for the application of WE as a chemopreventive functional food and as a prospective treatment of colon cancer.
Authors: Young Sun Lee; Jae Ha Yang; Man Jong Bae; Wang Keun Yoo; Shen Ye; Charlie C L Xue; Chun Guang Li Journal: Evid Based Complement Alternat Med Date: 2008-06-12 Impact factor: 2.629