Kuan-Ying Arthur Huang1, Jainn-Jim Lin2, Cheng-Hsun Chiu1, Shuan Yang3, Kuo-Chien Tsao3, Yhu-Chering Huang4, Tzou-Yien Lin5. 1. Department of Pediatrics, Chang Gung Children's Hospital Molecular Infectious Disease Research Centre. 2. Department of Pediatrics, Chang Gung Children's Hospital. 3. Department of Laboratory Medicine, Chang Gung Memorial Hospital. 4. Department of Pediatrics, Chang Gung Children's Hospital College of Medicine, Chang Gung University, Taoyuan, Taiwan. 5. College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Abstract
BACKGROUND: Enterovirus 71 (EV71) remains a leading pathogen for acute infectious diseases in children, especially in Asia. The cellular basis for establishing a virus-specific antibody response to acute EV71 infections is unclear in children. METHODS: We studied the magnitude of virus-specific antibody-secreting B cells (ASCs) and its relationship with serological response, clinical parameters, and virological parameters among children with laboratory-confirmed EV71 infection. RESULTS: A potent EV71 genogroup B- and virus-specific ASC response was detected in the first week of illness among genotype B5 EV71-infected children. The cross-reactive EV71-specific ASC response to genogroup C viral antigens composed about 10% of the response. The EV71-specific ASC response in children aged ≥3 years produced immunoglobulin G predominantly, but immunoglobulin M was predominant in younger children. Proliferation marker was expressed by the majority of circulating ASCs in the acute phase of EV71 infection. Virus-specific ASC responses significantly correlated with throat viral load, fever duration, and serological genogroup-specific neutralization titer. CONCLUSIONS: The presence of a virus-specific ASC response serves an early cellular marker of an EV71-specific antibody response. Further detailed study of EV71-specific ASCs at the monoclonal level is crucial to delineate the specificity and function of antibody immunity in children.
BACKGROUND:Enterovirus 71 (EV71) remains a leading pathogen for acute infectious diseases in children, especially in Asia. The cellular basis for establishing a virus-specific antibody response to acute EV71 infections is unclear in children. METHODS: We studied the magnitude of virus-specific antibody-secreting B cells (ASCs) and its relationship with serological response, clinical parameters, and virological parameters among children with laboratory-confirmed EV71infection. RESULTS: A potent EV71 genogroup B- and virus-specific ASC response was detected in the first week of illness among genotype B5 EV71-infected children. The cross-reactive EV71-specific ASC response to genogroup C viral antigens composed about 10% of the response. The EV71-specific ASC response in children aged ≥3 years produced immunoglobulin G predominantly, but immunoglobulin M was predominant in younger children. Proliferation marker was expressed by the majority of circulating ASCs in the acute phase of EV71infection. Virus-specific ASC responses significantly correlated with throat viral load, fever duration, and serological genogroup-specific neutralization titer. CONCLUSIONS: The presence of a virus-specific ASC response serves an early cellular marker of an EV71-specific antibody response. Further detailed study of EV71-specific ASCs at the monoclonal level is crucial to delineate the specificity and function of antibody immunity in children.
Authors: Michael J Carter; Ruth M Mitchell; Patrick M Meyer Sauteur; Dominic F Kelly; Johannes Trück Journal: Front Immunol Date: 2017-06-01 Impact factor: 7.561
Authors: Ling Zhu; Kangwei Xu; Nan Wang; Lei Cao; Junlan Wu; Qiang Gao; Elizabeth E Fry; David I Stuart; Zihe Rao; Junzhi Wang; Xiangxi Wang Journal: mBio Date: 2018-07-03 Impact factor: 7.867