Ewa Jaźwińska-Tarnawska1, Izabela Jęśkowiak2, Ewa Waszczuk3, Agata Mulak3, Krystyna Głowacka4, Magdalena Hurkacz2, Leszek Paradowski3, Zofia Zaleska2, Anna Wiela-Hojeńska5. 1. Department of Clinical Pharmacology Wroclaw Medical University, Wrocław, Poland. Electronic address: tje1@wp.pl. 2. Department of Clinical Pharmacology Wroclaw Medical University, Wrocław, Poland. 3. Department of Gastroenterology and Hepatology Wroclaw Medical University, Wrocław, Poland. 4. Department of Clinical Pharmacology Wroclaw Medical University, Wrocław, Poland. Electronic address: gastro@gastro.umed.wroc.pl. 5. Department of Clinical Pharmacology Wroclaw Medical University, Wrocław, Poland. Electronic address: wf-12@umed.wroc.pl.
Abstract
BACKGROUND: In recent years, an increasing incidence of inflammatory bowel disease (IBD) has been reported, mainly as Crohn's disease (CD) and ulcerative colitis (UC). The individual susceptibility, the disease's course and response to the applied therapy is likely due to genetic factors such as ABCB1 gene mutations, exemplified by C3435T polymorphism. The aim of the study was to evaluate the distribution of C3435T polymorphism regarding the gender in IBD patients and control subjects from Lower Silesia region and its possible association with IBD susceptibility. METHODS: The research was conducted in groups of 61 IBD patients and 101 healthy subjects from the Lower Silesia region. Polymorphism of C3435T was determined using PCR-RFLP method. RESULTS: Frequency distributions of C3435T genotype and of 3435T or 3435C gene alleles of IBD, CD or UC patients were compared to control group; each treated as a whole or split further by gender. The statistically significant correlation was discovered between gender and C3435T genotype both for IBD and CD patients, with 3435CT heterozygote prevailing in IBD and CD males. Odds ratio calculations revealed statistically significant difference for the 3435CT genotype between control and: IBD group considered as a whole; IBD males; CD males; and for 3435TT variant between control and IBD males. Conclusions. The 3435CT genotype could be a risk factor for IBD and CD in men. The 3435TT genotype in males seems to be associated with the lower chance of IBD presence.
BACKGROUND: In recent years, an increasing incidence of inflammatory bowel disease (IBD) has been reported, mainly as Crohn's disease (CD) and ulcerative colitis (UC). The individual susceptibility, the disease's course and response to the applied therapy is likely due to genetic factors such as ABCB1 gene mutations, exemplified by C3435T polymorphism. The aim of the study was to evaluate the distribution of C3435T polymorphism regarding the gender in IBD patients and control subjects from Lower Silesia region and its possible association with IBD susceptibility. METHODS: The research was conducted in groups of 61 IBD patients and 101 healthy subjects from the Lower Silesia region. Polymorphism of C3435T was determined using PCR-RFLP method. RESULTS: Frequency distributions of C3435T genotype and of 3435T or 3435C gene alleles of IBD, CD or UC patients were compared to control group; each treated as a whole or split further by gender. The statistically significant correlation was discovered between gender and C3435T genotype both for IBD and CDpatients, with 3435CT heterozygote prevailing in IBD and CD males. Odds ratio calculations revealed statistically significant difference for the 3435CT genotype between control and: IBD group considered as a whole; IBD males; CD males; and for 3435TT variant between control and IBD males. Conclusions. The 3435CT genotype could be a risk factor for IBD and CD in men. The 3435TT genotype in males seems to be associated with the lower chance of IBD presence.
Authors: Paweł Petryszyn; Robert Dudkowiak; Agnieszka Gruca; Ewa Jaźwińska-Tarnawska; Paweł Ekk-Cierniakowski; Elżbieta Poniewierka; Anna Wiela-Hojeńska; Krystyna Głowacka Journal: Genes (Basel) Date: 2021-09-15 Impact factor: 4.096