Literature DB >> 25712

Purification and some properties of Bacillus macerans cycloamylose (cyclodextrin) glucanotransferase.

S Kobayashi, K Kainuma, S Suzuki.   

Abstract

Bacillus macerans cycloamylose (cyclodextrin) glucanotransferase (EC 2.4.1.19) was purified by the technique of starch adsorption and DEAE-cellulose column chromatography, and then crystallized from an ammonium sulfate solution containing mM calcium chloride. The crystals of the enzyme were rod-shaped and showed a single band by disc-gel electrophorsis. The purified enzyme was dissociated into two subunits by sodium dodecyl sulfate-disc electrophoresis. The subunits had no enzyme activity. Details of each purification step and some properties of the enzyme are described in this paper.

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Year:  1978        PMID： 25712     DOI： 10.1016/s0008-6215(00)84484-5

Source DB:  PubMed          Journal:  Carbohydr Res        ISSN： 0008-6215            Impact factor:   2.104


  5 in total

1.  A super-secondary structure predicted to be common to several alpha-1,4-D-glucan-cleaving enzymes.

Authors:  E A MacGregor; B Svensson
Journal:  Biochem J       Date:  1989-04-01       Impact factor: 3.857

2.  Nucleotide sequence of the beta-cyclodextrin glucanotransferase gene of alkalophilic Bacillus sp. strain 1011 and similarity of its amino acid sequence to those of alpha-amylases.

Authors:  K Kimura; S Kataoka; Y Ishii; T Takano; K Yamane
Journal:  J Bacteriol       Date:  1987-09       Impact factor: 3.490

3.  Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene.

Authors:  T Takano; M Fukuda; M Monma; S Kobayashi; K Kainuma; K Yamane
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

4.  A novel synthesis method for cyclodextrins from maltose in water-organic solvent systems.

Authors:  T Morita; N Yoshida; I Karube
Journal:  Appl Biochem Biotechnol       Date:  1996-03       Impact factor: 2.926

5.  Role of Phe283 in enzymatic reaction of cyclodextrin glycosyltransferase from alkalophilic Bacillus sp.1011: Substrate binding and arrangement of the catalytic site.

Authors:  Ryuta Kanai; Keiko Haga; Toshihiko Akiba; Kunio Yamane; Kazuaki Harata
Journal:  Protein Sci       Date:  2004-02       Impact factor: 6.725
  5 in total

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