OBJECTIVE: The objectives of this study were to determine the association between single nucleotide polymorphisms (SNPs) in IL1B (-511, +3954), IL1A (-889, +4845), and the variable number of tandem repeats (VNTRs) polymorphism in the IL-1RN gene with chronic periodontitis susceptibility and to analyze gene-gene interactions in a hospital-based sample population from South India. SUBJECTS AND METHODS: A total of 400 individuals were recruited for this study; 200 individuals with healthy gingiva and 200 chronic periodontitis patients. Genomic DNA was isolated from peripheral blood samples and genotyping was performed for the above-mentioned single nucleotide and VNTR polymorphisms by polymerase chain reaction, DNA sequencing, and agarose gel electrophoresis. RESULTS: A higher proportion of the variant alleles were observed in the chronic periodontitis group for all the SNPs examined. The SNP at +3954 (C>T) in the IL1B gene was found to be significantly associated with chronic periodontitis (p=0.007). VNTR genotypes (χ(2) value: 5.163, df=1, p=0.023) and alleles (χ(2) value: 6.818, df=1, p=0.009) were found to have a significant association with chronic periodontitis susceptibility. CONCLUSION: In the study population examined, the SNP in the IL1B gene (+3954) and VNTR polymorphisms in the IL1RN gene were found to have a significant association with chronic periodontitis susceptibility.
OBJECTIVE: The objectives of this study were to determine the association between single nucleotide polymorphisms (SNPs) in IL1B (-511, +3954), IL1A (-889, +4845), and the variable number of tandem repeats (VNTRs) polymorphism in the IL-1RN gene with chronic periodontitis susceptibility and to analyze gene-gene interactions in a hospital-based sample population from South India. SUBJECTS AND METHODS: A total of 400 individuals were recruited for this study; 200 individuals with healthy gingiva and 200 chronic periodontitispatients. Genomic DNA was isolated from peripheral blood samples and genotyping was performed for the above-mentioned single nucleotide and VNTR polymorphisms by polymerase chain reaction, DNA sequencing, and agarose gel electrophoresis. RESULTS: A higher proportion of the variant alleles were observed in the chronic periodontitis group for all the SNPs examined. The SNP at +3954 (C>T) in the IL1B gene was found to be significantly associated with chronic periodontitis (p=0.007). VNTR genotypes (χ(2) value: 5.163, df=1, p=0.023) and alleles (χ(2) value: 6.818, df=1, p=0.009) were found to have a significant association with chronic periodontitis susceptibility. CONCLUSION: In the study population examined, the SNP in the IL1B gene (+3954) and VNTR polymorphisms in the IL1RN gene were found to have a significant association with chronic periodontitis susceptibility.
Authors: S P Engebretson; I B Lamster; M Herrera-Abreu; R S Celenti; J M Timms; A G Chaudhary; F S di Giovine; K S Kornman Journal: J Periodontol Date: 1999-06 Impact factor: 6.993
Authors: Y Ishihara; T Nishihara; T Kuroyanagi; N Shirozu; E Yamagishi; M Ohguchi; M Koide; N Ueda; K Amano; T Noguchi Journal: J Periodontal Res Date: 1997-08 Impact factor: 4.419
Authors: Josiane Bazzo de Alencar; Joana Maira Valentini Zacarias; Patrícia Yumeko Tsuneto; Victor Hugo de Souza; Cléverson de Oliveira E Silva; Jeane Eliete Laguila Visentainer; Ana Maria Sell Journal: PLoS One Date: 2020-01-24 Impact factor: 3.240
Authors: An Li; Renske Z Thomas; Luc van der Sluis; Geerten-Has Tjakkes; Dagmar Else Slot Journal: Int J Dent Hyg Date: 2020-05-24 Impact factor: 2.477