Cheng-Ye Che1, Jing-Fen Zhang2, Ji-Eun Lee3, Jing Lin1, Li-Ting Hu1, Nan Jiang1, Qian Wang1, Qiang Xu1, Gui-Qiu Zhao1. 1. Department of Ophthalmology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China. 2. Shandong Lunan Eye Hospital, Eye Hospital of Shandong Medical College, Linyi 276002, Shandong Province, China. 3. Department of Ophthalmology, School of Medicine, Pusan National University, Pusan 609-735, Korea.
Abstract
AIM: To evaluate the early expression of mannose-binding lectin 2 (MBL2) in human corneal epithelial cells (HCECs) infected by Aspergillus fumigatus (AF). METHODS: HCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h. The expression of MBL2 mRNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression of MBL2 protein in supernatant fluid was shown by enzyme linked immunosorbent assay (ELISA). MBL2 protein in HCECs was detected by immunocytochemistry at 0 and 24h. RESULTS: MBL2 mRNA and protein are expressed in normal HCECs. The expression of MBL2 mRNA and protein in supernatant fluid begin to increase after being stimulated with AF antigens. The most significantly peak of MBL2 mRNA is in 2h. The protein of MBL2 in supernatant fluid decrease gradually after 0.5h. The protein in HCECs expression increase after stimulation of 24h. CONCLUSION: MBL2 receptor expressed in normal HCECs in vitro. The stimulation by AF antigens can increase the early expression of it.
AIM: To evaluate the early expression of mannose-binding lectin 2 (MBL2) in human corneal epithelial cells (HCECs) infected by Aspergillus fumigatus (AF). METHODS: HCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h. The expression of MBL2 mRNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression of MBL2 protein in supernatant fluid was shown by enzyme linked immunosorbent assay (ELISA). MBL2 protein in HCECs was detected by immunocytochemistry at 0 and 24h. RESULTS:MBL2 mRNA and protein are expressed in normal HCECs. The expression of MBL2 mRNA and protein in supernatant fluid begin to increase after being stimulated with AF antigens. The most significantly peak of MBL2 mRNA is in 2h. The protein of MBL2 in supernatant fluid decrease gradually after 0.5h. The protein in HCECs expression increase after stimulation of 24h. CONCLUSION:MBL2 receptor expressed in normal HCECs in vitro. The stimulation by AF antigens can increase the early expression of it.
Entities:
Keywords:
Aspergillus fumigatus; human corneal epithelial cells; mannose-binding lectin
Authors: Steven de Jesus Carrion; Sixto M Leal; Mahmoud A Ghannoum; Vishukumar Aimanianda; Jean-Paul Latgé; Eric Pearlman Journal: J Immunol Date: 2013-08-07 Impact factor: 5.422