Literature DB >> 2570562

Detection of HIV-1 DNA in different subsets of human peripheral blood mononuclear cells using the polymerase chain reaction. Rapid communication.

F T Hufert1, D von Laer, C Schramm, A Tárnok, H Schmitz.   

Abstract

The presence of HIV-1 proviral DNA was determined in CD4 cells, CD8 cells and macrophages/monocytes obtained from peripheral blood of 8 HIV-1 infected persons. Using the polymerase chain reaction (PCR) we were able to detect proviral DNA in the extracts of only 10(2)-10(3) CD4 (T4) cells. In contrast, 10(5) CD8 cells did not contain detectable amounts of proviral DNA. Surprisingly, in four of our eight patients studied no HIV-1 DNA was found in macrophages. In peripheral blood, every hundred T4 cell may be infected with HIV-1.

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Year:  1989        PMID： 2570562     DOI： 10.1007/BF01313963

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  8 in total

1.  Direct detection of HIV RNA expression in seropositive subjects.

Authors:  C Hart; G Schochetman; T Spira; A Lifson; J Moore; J Galphin; J Sninsky; C Y Ou
Journal:  Lancet       Date:  1988-09-10       Impact factor: 79.321

2.  DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells.

Authors:  C Y Ou; S Kwok; S W Mitchell; D H Mack; J J Sninsky; J W Krebs; P Feorino; D Warfield; G Schochetman
Journal:  Science       Date:  1988-01-15       Impact factor: 47.728

3.  Two-color flow cytometric analysis of monocyte depleted human blood lymphocyte subsets.

Authors:  T A Fleisher; G E Marti; C Hagengruber
Journal:  Cytometry       Date:  1988-07

4.  Isolation of HIV-1 from monocytes but not T lymphocytes.

Authors:  M Popovic; S Gartner
Journal:  Lancet       Date:  1987-10-17       Impact factor: 79.321

5.  Enzymatic gene amplification: qualitative and quantitative methods for detecting proviral DNA amplified in vitro.

Authors:  M A Abbott; B J Poiesz; B C Byrne; S Kwok; J J Sninsky; G D Ehrlich
Journal:  J Infect Dis       Date:  1988-12       Impact factor: 5.226

6.  Detection of lymphocytes expressing human T-lymphotropic virus type III in lymph nodes and peripheral blood from infected individuals by in situ hybridization.

Authors:  M E Harper; L M Marselle; R C Gallo; F Wong-Staal
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

7.  Selective tropism of lymphadenopathy associated virus (LAV) for helper-inducer T lymphocytes.

Authors:  D Klatzmann; F Barré-Sinoussi; M T Nugeyre; C Danquet; E Vilmer; C Griscelli; F Brun-Veziret; C Rouzioux; J C Gluckman; J C Chermann
Journal:  Science       Date:  1984-07-06       Impact factor: 47.728

8.  Rapid and sensitive viral culture method for human immunodeficiency virus type 1.

Authors:  J B Jackson; R W Coombs; K Sannerud; F S Rhame; H H Balfour
Journal:  J Clin Microbiol       Date:  1988-07       Impact factor: 5.948
  8 in total
  12 in total

1.  Markers for HIV-disease progression in untreated patients and patients receiving AZT: evaluation of viral activity, AZT resistance, serum cholesterol, beta 2-microglobulin, CD4+ cell counts, and HIV antigen.

Authors:  H Rübsamen-Waigmann; B Schröder; L Biesert; C D Bauermeister; H von Briesen; H Suhartono; F Zimmermann; H D Brede; A Regeniter; S Gerte
Journal:  Infection       Date:  1991       Impact factor: 3.553

2.  Circulating monocytes are not a major reservoir of HIV-1 in elite suppressors.

Authors:  Adam M Spivak; Maria Salgado; S Alireza Rabi; Karen A O'Connell; Joel N Blankson
Journal:  J Virol       Date:  2011-07-27       Impact factor: 5.103

3.  Progression of HIV-1 infection. Monitoring of HIV-1 DNA in peripheral blood mononuclear cells by PCR.

Authors:  F T Hufert; D von Laer; T E Fenner; S Schwander; P Kern; H Schmitz
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

4.  Proviral burden and infection kinetics of feline immunodeficiency virus in lymphocyte subsets of blood and lymph node.

Authors:  G A Dean; G H Reubel; P F Moore; N C Pedersen
Journal:  J Virol       Date:  1996-08       Impact factor: 5.103

5.  Simian immunodeficiency virus infection of CD8+ lymphocytes in vivo.

Authors:  G A Dean; G H Reubel; N C Pedersen
Journal:  J Virol       Date:  1996-08       Impact factor: 5.103

6.  Detection of Lassa virus RNA in specimens from patients with Lassa fever by using the polymerase chain reaction.

Authors:  K Lunkenheimer; F T Hufert; H Schmitz
Journal:  J Clin Microbiol       Date:  1990-12       Impact factor: 5.948

7.  Attenuation of human immunodeficiency virus type 1 cytopathic effect by a mutation affecting the transmembrane envelope glycoprotein.

Authors:  M Kowalski; L Bergeron; T Dorfman; W Haseltine; J Sodroski
Journal:  J Virol       Date:  1991-01       Impact factor: 5.103

8.  Antibodies of symptomatic human immunodeficiency virus type 1-infected individuals are directed to the V3 domain of noninfectious and not of infectious virions present in autologous serum.

Authors:  M Schreiber; H Petersen; C Wachsmuth; H Müller; F T Hufert; H Schmitz
Journal:  J Virol       Date:  1994-06       Impact factor: 5.103

9.  Dissociation of unintegrated viral DNA accumulation from single-cell lysis induced by human immunodeficiency virus type 1.

Authors:  L Bergeron; J Sodroski
Journal:  J Virol       Date:  1992-10       Impact factor: 5.103

10.  Characterization of 20 Entamoeba histolytica strains isolated from patients with HIV infection.

Authors:  G D Burchard; F T Hufert; D Mirelman
Journal:  Infection       Date:  1991 May-Jun       Impact factor: 3.553
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