| Literature DB >> 25704089 |
Xia Jiang1, Tatsuo Kanda2, Shingo Nakamoto3, Kengo Saito4, Masato Nakamura1, Shuang Wu1, Yuki Haga1, Reina Sasaki1, Naoya Sakamoto5, Hiroshi Shirasawa4, Hiroaki Okamoto6, Osamu Yokosuka1.
Abstract
The JAK2 inhibitor AZD1480 has been reported to inhibit La protein expression. We previously demonstrated that the inhibition of La expression could inhibit hepatitis A virus (HAV) internal ribosomal entry-site (IRES)-mediated translation and HAV replication in vitro. In this study, we analyzed the effects of AZD1480 on HAV IRES-mediated translation and replication. HAV IRES-mediated translation in COS7-HAV-IRES cells was inhibited by 0.1-1 μM AZD1480, a dosage that did not affect cell viability. Results showed a significant reduction in intracellular HAV HA11-1299 genotype IIIA RNA levels in Huh7 cells treated with AZD1480. Furthermore, AZD1480 inhibited the expression of phosphorylated-(Tyr-705)-signal transducer and activator of transcription 3 (STAT3) and La in Huh7 cells. Therefore, we propose that AZD1480 can inhibit HAV IRES activity and HAV replication through the inhibition of the La protein.Entities:
Keywords: AZD1480; HAV; IRES; La; STAT3
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Year: 2015 PMID： 25704089 DOI： 10.1016/j.bbrc.2015.02.058
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575