Determination of eight pharmaceuticals in an aqueous sample using automated derivatization solid-phase microextraction combined with gas chromatography-mass spectrometry.

An automated aqueous derivatization solid-phase microextraction (SPME) coupled with a gas chromatography/mass spectrometry (GC/MS) method was developed for simultaneous determination of eight pharmaceuticals in water samples. Dimethyl sulfate and tetrabutylammonium hydrogen sulfate were selected as derivatization and activation reagents for the esterification reaction. An experimental design approach, central composition design (CCD), was employed to investigate and optimize the operative factors influencing the extraction efficiency, including extraction time, extraction temperature and ionic strength. The other parameters such as type of fiber coating, pH and derivatization conditions were also evaluated. SPME was finally carried out in headspace mode at 80°C for 60min with the presence of 3.00g Na2SO4, using a home-made 44μm PDMS fiber. Wide linear ranges and low limits of detection (0.06-1.24ng L(-1)) were obtained under the optimized conditions. The relative standard deviations (RSDs) and recoveries ranged from 0.5% to 12.3% and 85% to 110%, respectively. The proposed method was successfully applied to the analysis of the real surface water samples from the Pearl River Estuary.