| Literature DB >> 25702109 |
Simone Külzer1, Verena Bittl, Jude M Przyborski.
Abstract
Subcellular fractionation is a valuable tool to follow protein traffic between cellular compartments. Here we detail a procedure for fractionating erythrocytes infected with the human malaria parasite P. falciparum using the bacterial pore-forming protein Streptolysin O (SLO). Additionally we describe an experimental protocol to determine protein topology by carrying out a protease protection assay on SLO-lysed infected erythrocytes.Entities:
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Year: 2015 PMID： 25702109 DOI： 10.1007/978-1-4939-2309-0_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745