Ping Yu1, Si Cheng1, Juan Xiang1, Bin Yu1, Mian Zhang2, Chaofeng Zhang1, Xianghong Xu3. 1. Research Department of Pharmacognosy, China Pharmaceutical University, #639 Longmian Dadao, Nanjing 211198, China. 2. Research Department of Pharmacognosy, China Pharmaceutical University, #639 Longmian Dadao, Nanjing 211198, China. Electronic address: mianzhang@126.com. 3. Research Department of Pharmacognosy, China Pharmaceutical University, #639 Longmian Dadao, Nanjing 211198, China. Electronic address: xuxh168@126.com.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: The root of Aster tataricus L. f., recorded in all versions of Chinese Pharmacopoeia, is a traditional Chinese medicine with the function of dispelling phlegm and relieving cough for more than 2000 years. This study was designed to evaluate the expectorant, antitussive, and anti-inflammatory activities of the root of A. tataricus and to explore the chemical substances responsible for these activities. MATERIALS AND METHODS: The 70% ethanol extract of the root of A. tataricus (RA-70) was divided into three fractions, Fr-0, Fr-50 and Fr-95. They were all orally administrated to the mice to investigate their potential expectorant activities by a tracheal phenol red secretion method. The most effective fraction, together with shionone, was evaluated the expectorant, antitussive and anti-inflammatory activities by the mouse models of phenol red secretion, ammonia-induced cough, and xylene-induced ear swelling. Furthermore, the chemical components of the effective fraction were analyzed and identified by an HPLC-Q-TOF/MS method. RESULTS: Treatment with RA-70, Fr-0 and Fr-50 increased the amount of phenol red secretion by 65.3%, 56.5%, and 76.9%, respectively. Fr-50 was chosen for the further investigation and the results showed that Fr-50 at 40, 80 mg/kg significantly enhanced the phenol red secretion of tracheas, increased the latent period and decreased the frequency of cough and inhibited the ear edema in mice. Shionone at 80 mg/kg showed the trend of enhancing sputum secreting, but had no effect on ammonia-induced cough and xylene-induced ear edema. HPLC-Q-TOF/MS analysis indicated that Fr-50 was mainly composed of 12 caffeoylquinic acids (40.8%, in relative peak area), 7 astersaponins (12.0%) and 13 astins/asterinins (pentapeptides, 26.5%). CONCLUSIONS: The root of A. tataricus has significant expectorant, antitussive and anti-inflammatory effects. Caffeoylquinic acids, astersaponins, and aster peptides, rather than shionone, may be the main constituents responsible for the expectorant and antitussive activities of A. tataricus and act in a synergistic way.
ETHNOPHARMACOLOGICAL RELEVANCE: The root of Aster tataricus L. f., recorded in all versions of Chinese Pharmacopoeia, is a traditional Chinese medicine with the function of dispelling phlegm and relieving cough for more than 2000 years. This study was designed to evaluate the expectorant, antitussive, and anti-inflammatory activities of the root of A. tataricus and to explore the chemical substances responsible for these activities. MATERIALS AND METHODS: The 70% ethanol extract of the root of A. tataricus (RA-70) was divided into three fractions, Fr-0, Fr-50 and Fr-95. They were all orally administrated to the mice to investigate their potential expectorant activities by a tracheal phenol red secretion method. The most effective fraction, together with shionone, was evaluated the expectorant, antitussive and anti-inflammatory activities by the mouse models of phenol red secretion, ammonia-induced cough, and xylene-induced ear swelling. Furthermore, the chemical components of the effective fraction were analyzed and identified by an HPLC-Q-TOF/MS method. RESULTS: Treatment with RA-70, Fr-0 and Fr-50 increased the amount of phenol red secretion by 65.3%, 56.5%, and 76.9%, respectively. Fr-50 was chosen for the further investigation and the results showed that Fr-50 at 40, 80 mg/kg significantly enhanced the phenol red secretion of tracheas, increased the latent period and decreased the frequency of cough and inhibited the ear edema in mice. Shionone at 80 mg/kg showed the trend of enhancing sputum secreting, but had no effect on ammonia-induced cough and xylene-induced ear edema. HPLC-Q-TOF/MS analysis indicated that Fr-50 was mainly composed of 12 caffeoylquinic acids (40.8%, in relative peak area), 7 astersaponins (12.0%) and 13 astins/asterinins (pentapeptides, 26.5%). CONCLUSIONS: The root of A. tataricus has significant expectorant, antitussive and anti-inflammatory effects. Caffeoylquinic acids, astersaponins, and aster peptides, rather than shionone, may be the main constituents responsible for the expectorant and antitussive activities of A. tataricus and act in a synergistic way.
Authors: Thomas Schafhauser; Linda Jahn; Norbert Kirchner; Andreas Kulik; Liane Flor; Alexander Lang; Thibault Caradec; David P Fewer; Kaarina Sivonen; Willem J H van Berkel; Philippe Jacques; Tilmann Weber; Harald Gross; Karl-Heinz van Pée; Wolfgang Wohlleben; Jutta Ludwig-Müller Journal: Proc Natl Acad Sci U S A Date: 2019-12-06 Impact factor: 11.205
Authors: Gao Zhen; Wang Jing; Jing Jing; Dan Xu; Li Zheng; Fengsen Li Journal: Evid Based Complement Alternat Med Date: 2018-07-18 Impact factor: 2.629