Literature DB >> 25696225

Methods in molecular cardiology: suppression subtraction hybridisation.

M B Smeets, G Pasterkamp, D P V de Kleijn.   

Abstract

Identification of differentially expressed genes in pathological tissues may provide more insight into the molecular mechanism involved in the development or progression of disease. Suppression subtraction hybridisation (SSH) allows the identification of novel, differentially expressed genes without prior knowledge about gene expression patterns or functional and biochemical protein characteristics. This review provides a general overview of the SSH method and gives two examples of SSH in the cardiovascular field.

Keywords:  SSH library; differentially expressed genes; identification; suppression subtraction hybridisation

Year:  2003        PMID： 25696225      PMCID： PMC2499899     

Source DB:  PubMed          Journal:  Neth Heart J        ISSN： 1568-5888            Impact factor:   2.380


  7 in total

1.  DNA chip technology.

Authors:  K M Kurian; C J Watson; A H Wyllie
Journal:  J Pathol       Date:  1999-02       Impact factor: 7.996

2.  Combining SSH and cDNA microarrays for rapid identification of differentially expressed genes.

Authors:  G P Yang; D T Ross; W W Kuang; P O Brown; R J Weigel
Journal:  Nucleic Acids Res       Date:  1999-03-15       Impact factor: 16.971

3.  Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.

Authors:  L Diatchenko; Y F Lau; A P Campbell; A Chenchik; F Moqadam; B Huang; S Lukyanov; K Lukyanov; N Gurskaya; E D Sverdlov; P D Siebert
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-11       Impact factor: 11.205

4.  Fibrillar collagen specifically regulates human vascular smooth muscle cell genes involved in cellular responses and the pericellular matrix environment.

Authors:  T Ichii; H Koyama; S Tanaka; S Kim; A Shioi; Y Okuno; E W Raines; H Iwao; S Otani; Y Nishizawa
Journal:  Circ Res       Date:  2001-03-16       Impact factor: 17.367

5.  Gene-specific messenger RNA: isolation by the deletion method.

Authors:  E K Bautz; E Reilly
Journal:  Science       Date:  1966-01-21       Impact factor: 47.728

6.  Acute-phase protein haptoglobin is a cell migration factor involved in arterial restructuring.

Authors:  Dominique P V de Kleijn; Mirjam B Smeets; Patrick P C W Kemmeren; Sai K Lim; Ben J Van Middelaar; Evelyn Velema; Arjan Schoneveld; Gerard Pasterkamp; Cornelius Borst
Journal:  FASEB J       Date:  2002-05-21       Impact factor: 5.191

7.  Identification of genes potentially involved in rupture of human atherosclerotic plaques.

Authors:  B C Faber; K B Cleutjens; R L Niessen; P L Aarts; W Boon; A S Greenberg; P J Kitslaar; J H Tordoir; M J Daemen
Journal:  Circ Res       Date:  2001-09-14       Impact factor: 17.367
  7 in total

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