Amy L Sindler1, Kimberly Cox-York2, Lauren Reese2, Nathan S Bryan3, Douglas R Seals4, Christopher L Gentile5. 1. Department of Integrative Physiology, University of Colorado, Boulder, Boulder, CO, USA Department of Health and Human Physiology, University of Iowa, Iowa City, IA, USA. 2. Department of Food Science and Human Nutrition, Colorado State University, Fort Collins, CO, USA. 3. Texas Therapeutics Institute, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, TX, USA. 4. Department of Integrative Physiology, University of Colorado, Boulder, Boulder, CO, USA. 5. Department of Food Science and Human Nutrition, Colorado State University, Fort Collins, CO, USA Christopher.gentile@colostate.edu.
Abstract
AIM: We tested the hypothesis that short-term oral sodium nitrite supplementation would improve vascular dysfunction in obese, diabetic mice. METHODS AND RESULTS: Vascular function was determined in control mice and in db/db mice receiving drinking water with or without sodium nitrite (50 mg/L) for 5 weeks. Nitrite supplementation increased plasma nitrite concentrations in db/db mice (0.19±0.02 µM vs 0.80±0.26 µM; p < 0.05). Db/db mice had lower endothelium-dependent dilation (EDD) in response to increasing doses of acetylcholine versus heterozygous control mice (71.2% ± 14.3% vs 93% ± 7.0%; p < 0.05), and sodium nitrite supplementation restored endothelium-dependent dilation to control levels (92.9% ± 2.3% vs 93% ± 7.0%; p < 0.05). The improvement in endothelial function was accompanied by a reduction in intrinsic stiffness, but not by alterations in plasma or vascular markers of inflammation. CONCLUSION: These data suggest that sodium nitrite may be a novel therapy for treating diabetes-related vascular dysfunction; however, the mechanisms of improvement are unknown.
AIM: We tested the hypothesis that short-term oral sodium nitrite supplementation would improve vascular dysfunction in obese, diabeticmice. METHODS AND RESULTS: Vascular function was determined in control mice and in db/db mice receiving drinking water with or without sodium nitrite (50 mg/L) for 5 weeks. Nitrite supplementation increased plasma nitrite concentrations in db/db mice (0.19±0.02 µM vs 0.80±0.26 µM; p < 0.05). Db/db mice had lower endothelium-dependent dilation (EDD) in response to increasing doses of acetylcholine versus heterozygous control mice (71.2% ± 14.3% vs 93% ± 7.0%; p < 0.05), and sodium nitrite supplementation restored endothelium-dependent dilation to control levels (92.9% ± 2.3% vs 93% ± 7.0%; p < 0.05). The improvement in endothelial function was accompanied by a reduction in intrinsic stiffness, but not by alterations in plasma or vascular markers of inflammation. CONCLUSION: These data suggest that sodium nitrite may be a novel therapy for treating diabetes-related vascular dysfunction; however, the mechanisms of improvement are unknown.
Authors: Amy L Sindler; Bradley S Fleenor; John W Calvert; Kurt D Marshall; Melanie L Zigler; David J Lefer; Douglas R Seals Journal: Aging Cell Date: 2011-03-31 Impact factor: 9.304
Authors: Hong Jiang; Ashley C Torregrossa; Amy Potts; Dan Pierini; Mayank Aranke; Harsha K Garg; Nathan S Bryan Journal: Free Radic Biol Med Date: 2013-10-21 Impact factor: 7.376
Authors: Kevin G Soucy; Sungwoo Ryoo; Alexandre Benjo; Hyun Kyo Lim; Gaurav Gupta; Jayson S Sohi; Jeremy Elser; Miguel A Aon; Daniel Nyhan; Artin A Shoukas; Dan E Berkowitz Journal: J Appl Physiol (1985) Date: 2006-12
Authors: Nathan S Bryan; Bernadette O Fernandez; Selena M Bauer; Maria Francisca Garcia-Saura; Alexandra B Milsom; Tienush Rassaf; Ronald E Maloney; Ajit Bharti; Juan Rodriguez; Martin Feelisch Journal: Nat Chem Biol Date: 2005-09-18 Impact factor: 15.040
Authors: Bradley S Fleenor; Amy L Sindler; Jason S Eng; Devatha P Nair; R Blair Dodson; Douglas R Seals Journal: Exp Gerontol Date: 2012-05-12 Impact factor: 4.032
Authors: Wei Chih Ling; Dharmani Devi Murugan; Yeh Siang Lau; Paul M Vanhoutte; Mohd Rais Mustafa Journal: Sci Rep Date: 2016-09-12 Impact factor: 4.379