Marilin Rosa1, Hyo Sook Han2, Roohi Ismail-Khan2, Pushpa Allam-Nandyala2, Marilyn M Bui3. 1. Departments of Anatomic Pathology and Women's Oncology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA marilin.rosa@moffitt.org. 2. Women's Oncology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA. 3. Departments of Anatomic Pathology and Women's Oncology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA.
Abstract
BACKGROUND: β-catenin is a critical component of the cadherin cell-to-cell adhesion pathway and a key participant in the Wnt signaling pathway. Activation of β-catenin signaling in the Wnt pathway is a known contributor to tumor cancer progression and metastasis and may result in resistance to chemotherapeutic agents. The aim of this study is to evaluate the patterns of expression of β-catenin in breast carcinoma cells before and after neoadjuvant chemotherapy. Discovery of the molecular mechanisms responsible for resistance to chemotherapy treatment could result in more effective therapy, and improve outcome and survival. DESIGN: Twenty-nine matched pre-treatment and post-neoadjuvant chemotherapy breast carcinomas were subjected to immunohistochemical study using anti-β-catenin antibody. Normal staining was defined as crisp membrane staining in >90% tumor cells; aberrant expression was nuclear staining in >5% tumor cells. RESULTS: Of the 29 included cases, five cases of invasive lobular carcinoma lacked β-catenin immunoreactivity pre- and post-treatment. Mildly reduced membranous staining was seen in two post-treatment samples. One case of triple-negative ductal carcinoma had reduced pre- and post-treatment staining. All other cases showed normal pre- and post-treatment β-catenin expression. No aberrant staining was identified. CONCLUSION: In our study, there was no difference in the expression of β-catenin in pre- and post-neoadjuvant chemotherapy specimens. These results do not suggest that β-catenin plays a role in conferring neoadjuvant chemotherapy resistance.
BACKGROUND: β-catenin is a critical component of the cadherin cell-to-cell adhesion pathway and a key participant in the Wnt signaling pathway. Activation of β-catenin signaling in the Wnt pathway is a known contributor to tumor cancer progression and metastasis and may result in resistance to chemotherapeutic agents. The aim of this study is to evaluate the patterns of expression of β-catenin in breast carcinoma cells before and after neoadjuvant chemotherapy. Discovery of the molecular mechanisms responsible for resistance to chemotherapy treatment could result in more effective therapy, and improve outcome and survival. DESIGN: Twenty-nine matched pre-treatment and post-neoadjuvant chemotherapy breast carcinomas were subjected to immunohistochemical study using anti-β-catenin antibody. Normal staining was defined as crisp membrane staining in >90% tumor cells; aberrant expression was nuclear staining in >5% tumor cells. RESULTS: Of the 29 included cases, five cases of invasive lobular carcinoma lacked β-catenin immunoreactivity pre- and post-treatment. Mildly reduced membranous staining was seen in two post-treatment samples. One case of triple-negative ductal carcinoma had reduced pre- and post-treatment staining. All other cases showed normal pre- and post-treatment β-catenin expression. No aberrant staining was identified. CONCLUSION: In our study, there was no difference in the expression of β-catenin in pre- and post-neoadjuvant chemotherapy specimens. These results do not suggest that β-catenin plays a role in conferring neoadjuvant chemotherapy resistance.
Authors: Takashi Takeshita; Li Yan; Xuan Peng; Siker Kimbung; Thomas Hatschek; Ingrid A Hedenfalk; Omar M Rashid; Kazuaki Takabe Journal: Am J Cancer Res Date: 2020-08-01 Impact factor: 6.166